中文摘要：黄曲霉毒素（AF）是一种由曲霉产生的有害的次生代谢物。虽然AF的检测技术已经成熟，但尚需检测产AF菌的技术。为此，不断地改进平板培养法。然而，平板培养法存在着诸多问题，需要进一步细化。本研究中，将活性炭粉（碳）加入含有环糊精(CD)的培养基，以增强荧光对比度并提高AF产生菌的检测效率。结果发现加入碳后，检测到了数种在仅含CD的培养基中无法检测的真菌。碳也促进了马铃薯葡萄糖液体培养基中的真菌生长。说明培养基中加入碳能增强AF衍生荧光，利于观测。
外文摘要：Aflatoxin (AF) is a harmful secondary metabolite that is synthesized by the Aspergillus species. Although AF detection techniques have been developed, techniques for detection of AF synthetic fungi are still required. Techniques such as plate culture methods are continually being modified for this purpose. However, plate culture methods require refinement because they suffer from several issues. In this study, activated charcoal powder (carbon) was added to a culture medium containing cyclodextrin (CD) to enhance the contrast of fluorescence and improve the detection efficiency for AF synthetic fungi. Two culture media, potato dextrose agar and yeast extract sucrose agar, were investigated using both plate and liquid cultures. The final concentrations of CD and carbon in the media were 3 mg/mL and 0.3 mg/mL, respectively. Addition of carbon improved the visibility of fluorescence by attenuating approximately 30% of light scattering. Several fungi that could not be detected with only CD in the medium were detected with carbon addition. The carbon also facilitated fungal growth in the potato dextrose liquid medium. The results suggest that addition of carbon to media can enhance the observation of AF-derived fluorescence.
作者：aflatoxin；cyclodextrin；ultraviolet；carbon；plate culture Authors:Suzuki, T；Iwahashi, Y
作者单位：NARO
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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