中文摘要：人、动植物病原菌镰刀菌，产生大量的次生代谢物，造成全球性农产品污染，如经全面研究的脱氧雪腐镰刀菌烯醇（DON），能严重损害胃肠道屏障的完整性。然而，迄今为止，其它镰刀菌代谢物如恩镰孢菌素、白僵菌素、串珠镰刀菌素、组蛋白脱乙酰酶抑制剂、黄色镰刀菌素、镰刀菌丝红素、伊快霉素和比卡菌素的毒理人们知之甚少。为此，我们检查它们单独或群体与DON结合72小时以上对分化型肠上皮细胞(IPEC-J2)的肠屏障功能的作用。考虑到霉菌毒素污染的流行及与肠屏障损伤相关的疾病，本研究结果表明消费受污染的食品和饲料将严重影响健康。
外文摘要：The human, animal and plant pathogen Fusarium, which contaminates agricultural commodities worldwide, produces numerous secondary metabolites. An example is the thoroughly-investigated deoxynivalenol (DON), which severely impairs gastrointestinal barrier integrity. However, to date, the toxicological profile of other Fusarium-derived metabolites, such as enniatins, beauvericin, moniliformin, apicidin, aurofusarin, rubrofusarin, equisetin and bikaverin, are poorly characterized. Thus we examined their effects-as metabolites alone and as metabolites in combination with DON-on the intestinal barrier function of differentiated intestinal porcine epithelial cells (IPEC-J2) over 72 h. Transepithelial electrical resistance (TEER) was measured at 24-h intervals, followed by evaluation of cell viability using neutral red (NR) assay. Enniatins A, A1, B and B1, apicidin, aurofusarin and beauvericin significantly reduced TEER. Moniliformin, equisetin, bikaverin and rubrofusarin had no effect on TEER. In the case of apicidin, aurofusarin and beauvericin, TEER reductions were further substantiated by the addition of otherwise no-effect DON concentrations. In all cases, viability was unaffected, confirming that TEER reductions were not due to compromised viability. Considering the prevalence of mycotoxin contamination and the diseases associated with intestinal barrier disruption, consumption of contaminated food or feed may have substantial health implications.
外文关键词：emerging mycotoxin；transepithelial electrical resistance；IPEC-J2；deoxynivalenol；intestinal barrier function
作者：Springler, A；Vrubel, GJ；Mayer, E；Schatzmayr, G；Novak, B
作者单位：BIOMIN Res Ctr
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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