中文摘要：黄曲霉毒素，因对人畜具有遗传毒性及致癌性，是一类极其有害的化合物，常见于许多热带和亚热带地区。已证实紫外线（UV）照射是减少或消除黄曲霉毒素的有效方法。然而，UV照射黄曲霉毒素的降解产物及其安全性或毒性在实际生产如食食用油行业中尚不清楚。本研究用超高效液相色谱-四极杆精确聚焦质谱/质谱(UPLC-TQEF-MS/MS)分析了花生油中黄曲霉毒素B1（AFB1）的降解产物。本研究的结果为UV降解AFB1的代谢途径和危险性评估提供了新的资料。
外文摘要：Aflatoxins, a group of extremely hazardous compounds because of their genotoxicity and carcinogenicity to human and animals, are commonly found in many tropical and subtropical regions. Ultraviolet (UV) irradiation is proven to be an effective method to reduce or detoxify aflatoxins. However, the degradation products of aflatoxins under UV irradiation and their safety or toxicity have not been clear in practical production such as edible oil industry. In this study, the degradation products of aflatoxin B₁ (AFB₁) in peanut oil were analyzed by Ultra Performance Liquid Chromatograph-Thermo Quadrupole Exactive Focus mass spectrometry/mass spectrometry (UPLC-TQEF-MS/MS). The high-resolution mass spectra reflected that two main products were formed after the modification of a double bond in the terminal furan ring and the fracture of the lactone ring, while the small molecules especially nitrogen-containing compound may have participated in the photochemical reaction. According to the above results, the possible photodegradation pathway of AFB₁ in peanut oil is proposed. Moreover, the human embryo hepatocytes viability assay indicated that the cell toxicity of degradation products after UV irradiation was much lower than that of AFB₁, which could be attributed to the breakage of toxicological sites. These findings can provide new information for metabolic pathways and the hazard assessment of AFB₁ using UV detoxification.
外文关键词：aflatoxin B-1；photodegradation product；TQEF-MS/MS；cell viability；furan rings
作者：Mao, J；He, B；Zhang, LX；Li, PW；Zhang, Q；Ding, XX；Zhang, W
作者单位：Chinese Acad Agr Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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