中文摘要：食源性疾病致病菌日益增多，已经成为了全球重大的公共卫生问题，因此，迫切需要一种快速、简单、准确的细菌性病原体现场筛选方法。本文开发了一个便携式荧光生物传感系统，并对已爆发食源性疾病的三种主要细菌病原体进行了现场检测。本文开发的荧光纳米生物传感系统与基于纳米珠的免疫磁珠分离方法联用，对中国三个农业大省三个城市的农产品中大肠杆菌、李斯特菌和鼠伤寒沙门氏菌进行同时检测。特异性试验表明，该复合生物传感系统对食品样本中非靶细菌干扰较小，可在60分钟内完成采样，大肠杆菌、李斯特菌和鼠伤寒沙门氏菌在生菜、虾和牛肉中的检测限分别为102、103和103 CFU/mL。该便携式荧光生物传感系统可对食物中多种食源性病原菌进行快速现场检测，具有较大的潜力替代传统的ELISA 和PCR检测方法。
外文摘要：Foodborne diseases caused by pathogenic bacteria have increasingly
become a major public health issue worldwide. Rapid, simple, and
accurate detection methods are urgently needed for in-field screening of
bacterial pathogens. In our previous work, rapid detection methodologies
have been established based on fluorescent nanobiosensors for
simultaneous separation and detection of multiple foodborne pathogenic
bacteria. In this research, a portable fluorescent biosensing system was
designed and built and further assessed for in-field detection of three
main types of bacterial pathogens that have been associated with the
outbreaks of foodborne illness. Using the developed fluorescent
nanobiosensor coupled with nanobead-based immunomagnetic separation, we
conducted blind tests with the portable device to simultaneously detect
E. coli O157:H7, L. monocytogenes, and S. Typhimurium in different
*food* products in three cities selected from three big *agricultural*
provinces in China. Specificity tests showed low interference of this
multiplex biosensor from non-targets in *food* samples. The detection
could be done from sampling to results within 60 min. Limits of
detections of this method for E. coli O157:H7, L. monocytogenes, and S.
Typhimuriuin were determined to be 10(2), 10(3), and 10(3) CFU/mL in
lettuce, shrimp, and ground beef, respectively. Recovery tests were also
investigated and this method was evaluated to be accurate comparing with
the gold standard culturing method. Therefore, it is feasible for this
portable fluorescence biosensing system to be used in rapid and in-field
screening of multiple foodborne pathogenic bacteria in foods, such as
vegetables, livestock meat, and sea *food*. And together with
fluorescent nanobiosensors, it provides a promising alternative tool to
traditional culturing method, or even conventional ELISA and PCR based
methods. (C) 2016 Elsevier Ltd. All rights reserved.

 
外文关键词：Fluorescence, In-field, Multiplex detection, Foodborne pathogens, Portable device
作者：Xu, Lizhou; Lu, Zhan; Cao, Lulu; Pang, Haiying; Zhang, Qi; Fu, Yingchun; Xiong, Yonghua; Li, Yanyan; Wang, Xiaoyuan; Wang, Jianping; Ying, Yibin; Li, Yanbin
作者单位：Zhejiang Univ, C523 Nong Sheng Huan Bldg,Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China.
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：3
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