中文摘要：本文开发了一个用于检测南方番茄病毒的一步逆转录环介导等温扩增技术。该方法和常规RT-PCR技术具有相同的灵敏度，且不易被抑制剂干扰，该方法不仅成功地从番茄不同组织（叶、根、果实、种子）中检测出了南方番茄病毒，而且能从番茄植株提取物中成功地检测出南方番茄病毒，而传统RT-PCR技术则不能。因此，RT-LAMP技术是一种具有特异性、快速且成本低的检测方法，可应用于卫生项目实地调查。
 
外文摘要：Southern tomato virus (SW) is a double stranded RNA (dsRNA) virus
belonging to genus Amalgavirus (family Amalgamaviridae) which has been
detected in tomato plants showing stunting, *fruit* discoloration and
size reduction. A one-step reverse-transcription loop-mediated
isothermal amplification (RT-LAMP) assay was developed for the detection
of SW in total RNA or sap extracts (obtained just by grinding in buffer)
from SW-infected tomato plants by using a set of three primers pairs
which were designed to the sequence of the SW putative coat protein.
Amplification products were visualized by gel electrophoresis or direct
staining of DNA. The sensitivity of RT-LAMP was identical to that of the
conventional RT-PCR and less affected by the presence of polymerase
inhibitors. SW was detected by RT-LAMP in different tomato tissues, i.e.
leaves, roots, fruits and seeds. Also the virus was successfully
detected by RT-LAMP from sap extracts obtained from field tomato plants
whereas conventional RT-PCR did not. Results of this work show that
RT-LAMP is a specific, rapid and cheap procedure to detect SW and it
could be implemented on field surveys and sanitation programs. (C) 2016
Elsevier B.V. All rights reserved.
 
外文关键词：STV, Amalgamaviru, Plant virus, Solanum lycopersicum, Seed-borne virus
作者：Elvira-Gonzalez, L.; Puchades, A. V.; Carpino, C.; Alfaro-Fernandez, A.; Font-San-Ambrosio, M. I.; Rubio, L.; Galipienso, L.
作者单位：Inst Valenciano Invest Agrarias, Valencia 46113, Spain.
期刊名称：JOURNAL OF VIROLOGICAL METHODS
期刊影响因子：1.508
出版年份：2017
出版刊次：3
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