中文摘要：镰刀菌毒素脱氧雪腐镰刀菌烯醇（DON）具有不同的毒性效应，常见于小麦制品，在加工过程中保持稳定，对人畜构成了日益严重的健康问题。除母体化合物DON外，人畜暴露还包括乙酰化的真菌代谢物3-乙酰脱氧雪腐镰刀菌烯醇（3ADON）和15-乙酰脱氧雪腐镰刀菌烯醇（15ADON）及植物来源的DON-葡萄糖苷(DON3G)和细菌产生的脱环氧-DON(DOM-1)。本研究运用完善的Caco-2细胞模型对比这些自然形成的DON对细胞活性和屏障完整性标志物，以及促炎趋化因子CXC配体(CXCL8)的作用。结果显示，对于诱导屏障完整性的不利影响， 3ADON小于DON，而15ADON则大于DON。反之，DON3G和DOM-1没有表现出可测量的对屏障完整性的不利影响。也证明了低聚半乳糖(GOS)能保护上皮细胞免受DON及其乙酰化形式的毒害，说明GOS是保护人类免受DON及其衍生物毒害的有益的食品添加剂。
外文摘要：Considering the diverse toxic effects of the Fusarium toxin deoxynivalenol (DON), its common occurrence in wheat-based products, and its stability during processing, DON constitutes an increasing health concern for humans and animals. In addition to the parent compound DON, human and animal exposure encompasses the acetylated fungal metabolites 3-acetyl-deoxynivalenol (3ADON) and 15-acetyl-deoxynivalenol (15ADON) as well as the plant-derived DON-glucoside (DON3G) and the bacterial product de-epoxy-DON (DOM-1). In the current study we used the well-established Caco-2 cell model to compare the effects of these naturally occurring forms of DON on cell viability and markers of barrier integrity, as well as on the release of the pro-inflammatory chemokine chemokine CXC motif ligand (CXCL8). Results show that 3ADON is less potent in inducing adverse effects on barrier integrity when compared to DON, whereas 15ADON appears to be slightly more potent than DON. In contrast, DON3G and DOM-1 exerted no measurable adverse effects on the intestinal barrier. It was also demonstrated that galacto-oligosaccharides (GOS) are able to protect epithelial cells against DON and its acetylated forms, which suggests that GOS are beneficial food additives in the protection of vulnerable segments of the human population against adverse effects of DON and its derivatives.
外文关键词：mycotoxin；deoxynivalenol；3-acetyl-deoxynivalenol；15-acetyl-deoxynivalenol；de-epoxy-DON；DON-3-O-glucoside；intestinal barrier；CXCL8
作者：Alizadeh, A；Braber, S；Akbari, P；Kraneveld, A；Garssen, J；
作者单位：Univ Utrecht
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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