中文摘要：本研究旨在确认姜黄素(CM)是否能减轻黄曲霉毒素B1（AFB1）引起的肝毒性影响，并弄清鸡肝脏中改变细胞色素P450同工酶的表达是否参与这些影响的调节。研究发现，日粮中添加CM能保护AFB1引起的鸡肝损伤，可能是通过增强抗氧化能力协同抑制关键CYP450同工酶介导的将AFB1活化成有毒的AFB0来实现的。
外文摘要：This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B₁ (AFB₁)-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB₁ (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB₁ induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB₁ also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB₁-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB₁ into AFBO-including CYP1A1, CYP1A2, CYP2A6, and CYP3A4-were significantly induced in liver microsomes after 2-week exposure to AFB₁. These alterations induced by AFB₁ were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB₁-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB₁ to toxic AFBO.
外文关键词：curcumin；aflatoxin B-1；CYP450；AFBO-DNA；chicks
作者：Zhang, NY；Qi, M；Zhao, L；Zhu, MK；Guo, J；Liu, J；Gu, CQ；Rajput, SA；Krumm, CS；Qi, DS；Sun, LH
作者单位：Huazhong Agr Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
点击下载：姜黄素通过抑制小鸡肝脏中的细胞色素P450同功酶从而防止小鸡黄曲霉毒素B1肝中毒