中文摘要：胡椒碱是一种丰富的酰胺，从黑胡椒种子中提取，在体外对由某些化学致癌物和黄曲霉毒素B₁(AFB₁)引起的细胞毒性和遗传毒性致癌作用具有保护作用。本文通过微核和彗星试验，研究日粮中添加胡椒碱后，AFB1实验性中毒的肉鸡体内抗遗传毒性的能力。结果发现胡椒碱/AFB1组与对照组及与单独胡椒组之间无明显差异；日粮中添加60mg胡椒碱/kg饲料是安全的，有益于承受0.5mgAFB₁/kg体重的毒性作用的家禽的健康。
外文摘要：Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B₁ (AFB₁) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB₁, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg-1 feed, (A) 0.5 mg·AFB₁·kg-1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB₁. The experiment was conducted for 26 days. Chicks intoxicated with AFB₁ showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB₁ group. No significant differences were observed between the group piperine/AFB₁ and the control and piperine-alone groups. The addition 60 mg·kg-1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB₁·kg-1 body weight.
外文关键词：aflatoxin B1；broiler chicken；piperine；Aspergillus
作者：Cardoso, VD；Vermelho, AB；de Lima, CAR；de Oliveira, JM；de Lima, MEF；da Silva, LHP；Direito, GM；Danelli, MDM
作者单位：Univ Fed Rio de Janeiro
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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