中文摘要：采收前黄曲霉毒素污染（PAC）是一个全球花生生产的大问题。由无处不在的土壤真菌黄曲霉产生，黄曲霉毒素是自然发生的已知致癌物。真菌与寄主的相互作用使PAC情况很复杂，从而使PAC抗性育种进展缓慢。有研究表明花生成熟时干旱胁迫可以诱导黄曲霉毒素产生。本研究用自动防护装置控制根及PEG区的温、湿度，诱导黄曲霉毒素产生。用聚合酶链式反应(PCR)和高效液相色谱(HPLC)，选择符合下列条件的种子：感染了黄曲霉并受到黄曲霉毒素污染；及没受黄曲霉毒素污染。RNA测序分析揭示了描述受污染种子对比不受污染种子的转录状态的基因组。这些结果提示在受污染种子中改变了脂肪酸生物合成和脱落酸(ABA)信号并指出潜在的易感因子，ABR1，作为ABA信号受体可能
在PAC中发挥作用。
外文摘要：Pre-harvest aflatoxin contamination (PAC) is a major problem facing peanut production worldwide. Produced by the ubiquitous soil fungus, Aspergillus flavus, aflatoxin is the most naturally occurring known carcinogen. The interaction between fungus and host resulting in PAC is complex, and breeding for PAC resistance has been slow. It has been shown that aflatoxin production can be induced by applying drought stress as peanut seeds mature. We have implemented an automated rainout shelter that controls temperature and moisture in the root and peg zone to induce aflatoxin production. Using polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC), seeds meeting the following conditions were selected: infected with Aspergillus flavus and contaminated with aflatoxin; and not contaminated with aflatoxin. RNA sequencing analysis revealed groups of genes that describe the transcriptional state of contaminated vs. uncontaminated seed. These data suggest that fatty acid biosynthesis and abscisic acid (ABA) signaling are altered in contaminated seeds and point to a potential susceptibility factor, ABR1, as a repressor of ABA signaling that may play a role in permitting PAC.
外文关键词：Arachis hypogaea；Aspergillus；aflatoxin；RNA-seq；non-coding RNA；uHPLC
作者：Clevenger, J；Marasigan, K；Liakos, V；Sobolev, V；Vellidis, G；Holbrook, C；Ozias-Akins, P
作者单位：Univ Georgia
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
点击下载：RNA测序揭示采收前容许黄曲霉毒素污染的种子的状态并指出潜在的易感因子