中文摘要：黄曲霉毒素和黑色素是聚酮合成的产物。开展本研究旨在从真菌阻断色素沉着的化合物中查找可能抑制黄曲霉毒素B1(AFB1)生物合成的物质。检测了三种天然化合物（百里酚、3-羟基苯甲醛、康帕丁）和一种合成化合物（弗康唑）共四种物质阻断黄曲霉色素沉着和产AFB1的能力。所有化合物均能抑制固体培养基中真菌菌丝体的色素沉着。同时，百里酚、3-羟基苯甲醛、弗康唑刺激黄曲霉深层发酵培养液中AFB1的积累，而添加2.5μg/mL康帕丁后，AFB1减少了50倍。此外，康帕丁也能抑制固体培养基中黄曲霉孢子的形成。用康帕丁（50μg/g谷粒）体内处理玉米和小麦粒，AFB1积累分别减少了14和15倍。讨论了康帕丁作为潜在的AFB1抑制剂的进一步研究展望。
外文摘要：Aflatoxins and melanins are the products of a polyketide biosynthesis. In this study, the search of potential inhibitors of the aflatoxin B1 (AFB1) biosynthesis was performed among compounds blocking the pigmentation in fungi. Four compounds-three natural (thymol, 3-hydroxybenzaldehyde, compactin) and one synthetic (fluconazole)-were examined for their ability to block the pigmentation and AFB1 production in Aspergillus flavus. All compounds inhibited the mycelium pigmentation of a fungus growing on solid medium. At the same time, thymol, fluconazole, and 3-hydroxybenzaldehyde stimulated AFB1 accumulation in culture broth of A. flavus under submerged fermentation, whereas the addition of 2.5 μg/mL of compactin resulted in a 50× reduction in AFB1 production. Moreover, compactin also suppressed the sporulation of A. flavus on solid medium. In vivo treatment of corn and wheat grain with compactin (50 μg/g of grain) reduced the level of AFB1 accumulation 14 and 15 times, respectively. Further prospects of the compactin study as potential AFB1 inhibitor are discussed.
外文关键词：aflatoxin B1；fungal melanins；polyketide biosynthesis；compactin；thymol；fluconazole；3-hydroxybenzaldehyde；Aspergillus flavus
作者：Dzhavakhiya, VG；Voinova, TM；Popletaeva, SB；Statsyuk, NV；Limantseva, LA；Shcherbakova, LA
作者单位：All Russian Res Inst Phytopathol
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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