中文摘要：2012年从韩国三个薏苡产区（涟川郡、和顺郡和阴城郡）采集种子样品。在采集的所有样本中，用标准的吸水纸培养和琼脂试管法检测了400粒种子中真菌发生的情况，并检测了真菌的不同分类群。检测到的真菌中最主要的有镰刀菌、茎点菌、交链孢霉、枝孢菌、弯孢霉、旋孢腔菌和小光壳菌，其中镰刀菌占45.6%。并通过基于两个蛋白编码基因(EF-1α和β-微管蛋白)组合序列的系统发育分析，表征了10种镰刀菌，即F. incarnatum (11.67%)、F. kyushuense (10.33%)、水稻恶苗病菌(8.67%)、F. concentricum (6.00%)、镰孢菌亚种(5.67%)、禾谷镰刀菌（1.67%）、F. miscanthi (0.67%)、F. polyphialidicum (0.33%)、F. armeniacum (0.33%)和F. thapsinum (0.33%)。随后通过检测镰刀菌的形态特征进行确定。形态学观察结果与分子鉴定相吻合。用ELISA定量分析了菌株产伏马菌素(FUM)和玉米赤霉烯酮(ZEN)的能力，结果显示FUM仅由水稻恶苗菌产生，而ZEN则由镰孢菌亚种和禾谷镰刀菌产生。
外文摘要：Adlay seed samples were collected from three adlay growing regions (Yeoncheon, Hwasun, and Eumseong region) in Korea during 2012. Among all the samples collected, 400 seeds were tested for fungal occurrence by standard blotter and test tube agar methods and different taxonomic groups of fungal genera were detected. The most predominant fungal genera encountered were Fusarium, Phoma, Alternaria, Cladosporium, Curvularia, Cochliobolus and Leptosphaerulina. Fusarium species accounted for 45.6% of all species found; and, with phylogenetic analysis based on the combined sequences of two protein coding genes (EF-1α and β-tubulin), 10 Fusarium species were characterized namely, F. incarnatum (11.67%), F. kyushuense (10.33%), F. fujikuroi (8.67%), F. concentricum (6.00%), F. asiaticum (5.67%), F. graminearum (1.67%), F. miscanthi (0.67%), F. polyphialidicum (0.33%), F. armeniacum (0.33%), and F. thapsinum (0.33%). The Fusarium species were then examined for their morphological characteristics to confirm their identity. Morphological observations of the species correlated well with and confirmed their molecular identification. The ability of these isolates to produce the mycotoxins fumonisin (FUM) and zearalenone (ZEN) was tested by the ELISA quantitative analysis method. The result revealed that FUM was produced only by F. fujikuroi and that ZEN was produced by F. asiaticum and F. graminearum.
外文关键词：adlay seeds；ELISA；Fusarium；morphological data analysis；mycotoxins；phylogenetic analysis
作者：An, TJ；Shin, KS；Paul, NC；Kim, YG；Cha, SW；Moon, Y；Yu, SH；Oh, SK
作者单位：NIHHS
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：11
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