中文摘要：黄曲霉毒素是霉菌型毒素，主要由黄曲霉产生，是食品和粮食的常见污染物，在全球造成严重的经济和健康问题。为了找到去除或解毒这些毒素的有效细菌，本研究利用香豆素为唯一碳源的培养基，从土壤样品中分离到了一株能降解黄曲霉毒素B1（AFB1）的细菌。该菌株降解黄曲霉毒素B1的活性主要表现在无细胞上清液中，且这种活性具有热稳定性但对蛋白酶K敏感，表明对AFB1起作用的是胞外蛋白或酶。此外，用液相色谱-质谱联用（LC-MS）分析没有检测到AFB1的降解产物，表明母体AFB1可能经生物转化生成了化学性质不同于AFB1的化合物。
外文摘要：Aflatoxins are type of mycotoxins mainly produced by Aspergillus flavus and a common contaminant of food and grain, posing a serious economic and health problem worldwide. In order to find efficient bacteria to remove or detoxify these mycotoxins, a bacterial strain capable of degrading aflatoxin B1 (AFB1) was isolated from soil samples using a culture medium containing coumarin as the sole carbon source. Based on 16S rRNA gene sequence analysis, this isolate was identified as Bacillus subtilis JSW-1; its further characterization showed that it could inhibit the growth of A. flavus with an inhibition ratio of 58.3% and could degrade AFB1 by 67.2% after incubation at 30 °C for 72 h. The aflatoxin B1-degrading activity of isolate JSW-1 was predominantly attributed to the cell-free supernatant and this activity was found to be heat stable but sensitive to proteinase K treatment, indicating that the extracellular proteins or enzymes are responsible for the AFB1 degradation. In addition, no degradation products of AFB1 could be detected by liquid chromatography-mass spectrometry (LC-MS) analysis, indicating that the parent AFB1 might be biotransformed to compounds with chemical properties different from that of AFB1.
外文关键词：Mycotoxin；Aflatoxin B-1；Biodegradation；Bacillus subtilis
作者：Xia, XS；Zhang, Y；Li, MY；Garba, B；Zhang, Q；Wang, Y；Zhang, HY；Li, PW
作者单位：Jiangsu Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：5
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