中文摘要：镰刀菌毒素如单端孢霉烯族毒素、玉米赤霉烯酮和伏马菌素，存在于全球的谷物、动物饲料和牧草中。产业和监管部门需要低成本的实用方法用来测定样品中的多种霉菌毒素。本文研究了开发新的同时半定量测定玉米赤霉烯酮（ZEA）、T2毒素（T2）和伏马菌素B1（FUM）三种监管毒素的复合纳米阵列的可行性。此外，由于所使用抗体的交叉反应,该阵列也可检测HT2毒素及伏马菌素B2和B3。研究证明了在96孔微量板中该纳米阵列作为一个易于使用的灵敏的筛查工具，可以用于三种监管毒素的多重检测。还需要为终端新手用户改进自动化图像和数据分析软件，提高分析的整体速度。
外文摘要：Fusarium mycotoxins such as trichothecenes, zearalenone and fumonisins occur on a worldwide basis in cereal grains, animal feeds and forages. Practical solutions for multiple mycotoxin determination in samples are required by industry and regulators for cost effective screening purposes. The feasibility of developing a novel multiplex nanoarray for the simultaneous and semi-quantitative detection of three regulated mycotoxins: zearalenone (ZEA), T2-toxin (T2) and fumonisin B1 (FUM) was examined. Additionally, the assay was also able to detect HT2 toxin and fumonisin B2 and B3 due to the cross reactivity profiles of the antibodies used. Individual mycotoxin conjugates specific to the three mycotoxins were nano-spotted onto wells of a microtitre plate. Optimisation of assay parameters and antibodies was undertaken with both individual and multiplex calibration curves generated. A competitive assay format was employed enabling a calibration curve for concentration analysis and duplicate results for up to 40 samples in 70 min for the three target mycotoxins. The characteristics and performance of the nanoarray were evaluated including sensitivity and specificity for each target. Additionally, intra and inter spotting precision, cross reactivity, matrix effects and sample analysis in maize and wheat (n=8) was performed. Sensitivity, determined as the concentration causing 50% inhibition, was 70.1, 2.8 and 90.9 ppb in PBS, 172.4, 3.2 and 129.3 ppb in methanol, 197.4, 0.7 and 216.7 ppb in wheat and 43.6, 0.5 and 25.9 ppb in maize for ZEA, T2 and FUM respectively. Intra spotting precision was 6%, 11% and 10% for PBS and 5%, 11% and 12% for methanol for ZEA, T2 and FUM respectively. Inter spotting precision was 4%, 14% and 6% for PBS and 3%, 9% and 16% for methanol for ZEA, T2 and FUM respectively. The feasibility of the nanoarray as an easy to use sensitive screening tool in the 96 well format has been demonstrated for the multiplex detection of three regulated mycotoxins. Improvements in automated image and data analysis software for novice end users are required to improve the overall rapidity of analysis.
外文关键词：Mycotoxin；Nanoarray；Multiplex；Zearalenone；T2-toxin；Fumonisin
作者：McNamee, SE；Bravin, F；Rosar, G；Elliott, CT；Campbell, K
作者单位：Queens Univ
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：3
点击下载：一种同时快速检测玉米赤霉烯酮、T2毒素及伏马菌素的纳米阵列的开发