中文摘要：本文评估了量子点（QDS）和胶体金纳米粒子（CG）这两种检测谷物基质中的脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）和T2/HT2毒素(T2/HT2)的多重流式免疫分析(LFIA)标记。开发的两种分析方法均基于相同的免疫试剂（标记除外），因此可以客观地比较它们的分析特性。结果表明两种LFIAs都能用作现场同时检测小麦中DON、ZEN和HT2/T2的快速、高性价比和方便的定性工具，而无需特殊仪器。不过，基于QD的LFIA消耗的免疫试剂量更少且更加灵敏、经济；而结果更容易解释，假阴性率较低。
外文摘要：Quantum dots (QDs) and colloidal gold nanoparticles (CG) were evaluated as labels for multiplex lateral flow immunoassay (LFIA) for determination of mycotoxins deoxynivalenol (DON), zearalenone (ZEN) and T2/HT2-toxin (T2/HT2) in cereal matrices. Both developed assays were based on the same immunoreagents (except for the labels), therefore their analytical characteristics could be objectively compared. For both LFIAs antigens (DON-ovalbumin (OVA), ZEN-OVA and T2-OVA) and rabbit anti-mouse immunoglobulin were immobilized on a nitrocellulose membrane as three test lines and one control line, respectively. Depending on the LFIA, monoclonal antibodies (mAb) against DON, ZEN and T2 were conjugated with CdSeS/ZnS QDs or CG. T2 and HT2 were detected by one test line (T2-OVA) with an anti-T2 mAb which showed 110% cross-reactivity with HT2. Both tests were developed in accordance with the legal limits and were developed in such a way that they had the same cut-off limits of 1000 μg kg−1, 80 μg kg−1 and 80 μg kg−1 for DON, ZEN and T2/HT2, respectively in order to allow a correct comparison. Applicability of these assays was demonstrated by analysis of naturally contaminated wheat samples. The results demonstrate that both the LFIAs can be used as rapid, cost-effective and convenient qualitative tool for on-site screening for simultaneous detection of DON, ZEN and HT2/T2 in wheat without special instrumentation. However, the QD-based LFIA consumed less immunoreagents and was more sensitive and economically beneficial. In addition, the results were easier to interpret, resulting in a lower false negative rate.
外文关键词：Quantum dots；Colloid gold nanoparticle；Bioconjugation；Multiplex lateral flow immunoassay；Mycotoxin
作者：Foubert, A；Beloglazova, NV；De Saeger, S
作者单位：Univ Ghent
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：4.712
出版年份：2017
出版刊次：2
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