中文摘要：本文旨在研究用多次再生的免疫亲和柱(IAC)检测花生软糖中的黄曲霉毒素B1、 B2、G1和G2的可行性。结果发现用于每种黄曲霉毒素的重复使用次数：AFB1、AFB2、AFG1和AFG2分别为9、6、6和0。根据AOAC方法的性能标准，在本研究所用的加标水平下，70%到125%的回收率是可以接受的。
外文摘要：The aim of this study was to investigate the feasibility of using multitime-regenerated immunoaffinity column (IAC) for aflatoxins B1, B2, G1 and G2 in peanut confection. After each use, the IAC was washed immediately with phosphate-buffered saline and stored for >12 h prior to reuse. The evaluation procedure consisted of using extracts of naturally contaminated peanut confection (4 replicates), aflatoxin-free peanut confection (duplicates), and aflatoxin-free peanut confection sample spiked with the 4 aflatoxins (AFT) at 3 levels in 4 replicates. Each day, 18 test extracts were analyzed using 18 designated IACs. After each use, the IACs were regenerated and reused for corresponding test extracts on the following day. This procedure was repeated daily over the course of 9 days. Analytical steps included passing the test extracts through the IACs, washing the columns with water, and eluting AFT with methanol. The eluates were diluted with water and were subjected to reversed phase LC separation, post-column photochemical derivatization and fluorescence detection. After eluting AFT, IACs were immediately regenerated by washing with phosphate buffer solution and storing overnight at 8 °C for re-use the following day. Results were analyzed using ANOVA and Tukey tests. The numbers of reuse varied for each AF: For AFB1 AFB2, AFG1 and AFG2 could be reused for 9, 6, 6 and 0 times, respectively. According to AOAC method performance criteria, recoveries ranging from 70% to 125% are considered acceptable at the spiking levels used in this study.
外文关键词：Aflatoxins；Peanut confection "pacoca"；Immunoaffinity column；Regeneration
作者：Iha, MH；Mini, CA；Okada, IA；Briganti, RD；Trucksess, MW
作者单位：Adolfo Lutz Inst Ribeirao Preto VI
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：2
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