中文摘要：黄曲霉毒素是黄曲霉和寄生曲霉产生的高致癌、致畸、致病的次生代谢物，造成多种主食如花生、玉米和坚果污染。本研究从发酵大豆酱，一种中国传统食品中筛选并鉴定鲁氏酵母，通过好氧固态发酵降解花生粕中的黄曲霉毒素B1（AFB1）。据我们所知，这是首次报道鲁氏酵母能降解AFB1。
外文摘要：Aflatoxins are highly carcinogenic, teratogenetic, and morbigenous secondary metabolites of Aspergillus flavus and A. parasiticus that can contaminate multiple staple foods, such as peanut, maize, and tree nuts. In this study, Zygosaccharomyces rouxii was screened out and identified from fermented soy paste-one kind of traditional Chinese food-to detoxify aflatoxin B₁ (AFB₁) by aerobic solid state fermentation in peanut meal. The optimal degradation condition was chosen from single factor experiment, and the most effective detoxification rate was about 97%. As for liquid fermentation, we tested the binding ability of Z. rouxii, and the highest binding rate reached was 74.3% (nonviable cells of Z. rouxii) in phosphate-buffered saline (PBS). Moreover, the biotransformation of AFB₁ through fermentation of Z. rouxii in peanut meal was further verified by liquid chromatography/mass spectrometry (LC/MS). According to TIC scan, after fermentation by Z. rouxii, the AFB₁ in peanut meal was prominently degraded to the lowering peaks of AFB₁. Additionally, m/s statistics demonstrated that AFB₁ may be degraded to some new products whose structural properties may be different from AFB₁, or the degradation products may be dissolved in the aqueous phase rather than the organic phase. As far as we know, this is the first report indicating that the safe strain of Z. rouxii has the ability to detoxify AFB₁.
外文关键词：aflatoxin B-1；detoxification；Zygosaccharomyces rouxii；solid state fermentation；LC/MS
作者：Zhou, GH；Chen, YJ；Kong, Q；Ma, YX；Liu, Y
作者单位：Ocean Univ China
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：1
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