中文摘要：环匹阿尼酸（α-环匹阿尼酸，α-CPA）是一种由包括臭名昭著的污染食品和饲料的黄曲霉在内的多种真菌产生的吲哚茚烷特特拉姆酸神经毒素。虽然大约40年前就在黄曲霉培养基中发现了它，但对它的研究却越来越少，这是由于我们把研究重点放在了该菌产生的致癌性更强的黄曲霉毒素上面。本文筛选和鉴定了几种黄曲霉培养基中以前不曾发现的CPA型生物碱。黄曲霉培养基中明显存在的CPA及其众多衍生物引起了关于这些真菌次生代谢物长期积累的毒性效应以及它们对黄曲霉整体毒素问题的贡献的关注。
外文摘要：Cyclopiazonic acid (α-cyclopiazonic acid, α-CPA) is an indole-hydrindane-tetramic acid neurotoxin produced by various fungal species, including the notorious food and feed contaminant Aspergillus flavus. Despite its discovery in A. flavus cultures approximately 40 years ago, its contribution to the A. flavus mycotoxin burden is consistently minimized by our focus on the more potent carcinogenic aflatoxins also produced by this fungus. Here, we report the screening and identification of several CPA-type alkaloids not previously found in A. flavus cultures. Our identifications of these CPA-type alkaloids are based on a dereplication strategy involving accurate mass high resolution mass spectrometry data and a careful study of the α-CPA fragmentation pattern. In total, 22 CPA-type alkaloids were identified in extracts from the A. flavus strains examined. Of these metabolites, 13 have been previously reported in other fungi, though this is the first report of their existence in A. flavus. Two of our metabolite discoveries, 11,12-dehydro α-CPA and 3-hydroxy-2-oxo CPA, have never been reported for any organism. The conspicuous presence of CPA and its numerous derivatives in A. flavus cultures raises concerns about the long-term and cumulative toxicological effects of these fungal secondary metabolites and their contributions to the entire A. flavus mycotoxin problem.
外文关键词：cyclopiazonic acid；ergot-like alkaloid；dereplication；HRMS
作者：Uka, V；Moore, GG；Arroyo-Manzanares, N；Nebija, D；De Saeger, S；Di Mavungu, JD
作者单位：Univ Ghent
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：1
点击下载：用UHPLC三-TOF质谱揭示曲霉菌环匹阿尼酸家族毒素的多样性