中文摘要：玉米赤霉烯酮（ZEN）是一种雌激素毒素，常见于食品及饲料产品，影响人畜生殖和发育。本研究旨在弄清ZEN对SD鼠母体及其雌性F1的毒性作用。研究表明雌鼠产前ZEN暴露影响母体及胎儿发育，并可能导致F1代成年雌鼠长期的生殖障碍。
外文摘要：Zearalenone (ZEN) is an oestrogenic mycotoxin commonly found in food and feed products and can affect reproduction and development in both humans and animals. This study aimed to determine the toxic effects of ZEN on maternal SD rats and the F1 female offspring. Sixty-four pregnant rats were divided into 4 groups and exposed to feed contaminated with ZEN (0, 5, 10, and 20 mg/kg feed) on gestational days (GDs) 0-21. Compared with the controls, the groups exposed to 10 and 20 mg/kg ZEN showed significantly decreased feed intake and body weight of pregnant rats and/or female offspring. Meanwhile, 20 mg/kg ZEN significantly decreased the birth weight and viability of F1 newborn rats. Moreover, 10 and 20 mg/kg ZEN diets increased follicle-stimulating hormone concentrations but decreased oestradiol in both maternal and F1 adult rats. In the F1 generation, ZEN caused no pathological changes in ovaries and uterus in weaned rats, but significant follicular atresia and a thinning uterine layer were found in F1 female adult rats in the 20 mg/kg ZEN group. These impairments concurred with the inhibited mRNA and protein levels of oestrogen receptor-alpha (Esr1) and 3β-hydroxysteroid dehydrogenase (HSD) in the adult uterus and/or ovaries. Furthermore, 10 and/or 20 mg/kg ZEN exposure significantly reduced Esr1, gonadotropin-releasing hormone receptor (GnRHr), and ATP binding cassette transporters b1 and c1 (ABCb1 and ABCc1) in the placenta and foetal and weaned F1 brains, and also produced a dose-dependent increase in 3β-HSD in the placenta. Additionally, 20 mg/kg ZEN significantly upregulated ABCc5 expression in the placenta and ovaries of weaned rats. These results suggested that prenatal ZEN exposure in rats affected maternal and foetal development and may lead to long-term reproductive impairment in F1 adult females.
外文关键词：zearalenone；gestational exposure；transgenerational toxicity；F1 female rats
作者：Gao, X；Sun, LH；Zhang, NY；Li, C；Zhang, JC；Xiao, ZH；Qi, DS
作者单位：Huazhong Agr Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：1
点击下载：孕期玉米赤霉烯酮暴露引起孕鼠及其雌性后代生殖和发育的毒性