中文摘要：黄曲霉毒素B１（AFB１）是一种常见的霉菌毒素，由产毒曲霉产生。据报道AFB１能引起人畜严重的不良健康作用，如癌症和异常生殖与发育。AFB１也是一种有效的遗传毒性诱变剂，引起体内外DNA损伤。然而，DNA损伤与异常生殖发育间的关系尚不清楚。为解决这一问题，本文以秀丽线虫为模型，研究了AFB1暴露后的DNA损伤、生殖细胞凋亡、生长和生殖毒性。结果发现AFB1以浓度依赖方式诱导DNA损伤和生殖细胞凋亡。AFB1暴露对DNA修复缺陷的xpa-1线虫的生长或生殖抑制更甚于其野生型N2。据实时PCR测得的路径相关基因的相对表达水平，发现DNA损伤响应（DDR）路径与AFB1诱导的生殖细胞凋亡相关，而后者进一步加重了线虫的生长和生殖功能障碍。
外文摘要：Aflatoxin B₁ (AFB₁) is a ubiquitous mycotoxin produced by toxicogenic Aspergillus species. AFB₁ has been reported to cause serious adverse health effects, such as cancers and abnormal development and reproduction, in animals and humans. AFB₁ is also a potent genotoxic mutagen that causes DNA damage in vitro and in vivo. However, the link between DNA damage and abnormal development and reproduction is unclear. To address this issue, we examined the DNA damage, germline apoptosis, growth, and reproductive toxicity following exposure to AFB₁, using Caenorhabditis elegans as a study model. Results found that AFB₁ induced DNA damage and germline apoptosis, and significantly inhibited growth and reproduction of the nematodes in a concentration-dependent manner. Exposure to AFB₁ inhibited growth or reproduction more potently in the DNA repair-deficient nematodes than the wild-type N2 strain. According to the relative expression level of pathway-related genes measured by real-time PCR, the DNA damage response (DDR) pathway was found to be associated with AFB₁-induced germline apoptosis, which further played an essential role in the dysfunction of growth and reproduction in C. elegans.
外文关键词：Aflatoxin B-1；apoptosis；Caenorhabditis elegans；DNA damage response；growth and reproductive toxicity
作者：Feng, WH；Xue, KS；Tang, LL；Williams, PL；Wang, JS
作者单位：Univ Georgia
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：1
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