中文摘要：本研究中，分子表征了20种最常见镰刀菌并将它们接种到马铃薯葡萄糖琼脂(PDA)、水稻玉米培养基上，用液相色谱-串联质谱(LC-MS/MS)检测到了33种目标霉菌毒素，可能是所鉴定真菌的次生代谢物。本研究首次得到了一个全面显示所选真菌在各种基质中产生霉菌毒素的模式图解，因而从应用角度为探索镰刀菌的产毒能力及预测镰刀菌侵染/霉菌毒素污染提供了关键资料。
外文摘要：In this study, twenty of the most common Fusarium species were molecularly characterized and inoculated on potato dextrose agar (PDA), rice and maize medium, where thirty three targeted mycotoxins, which might be the secondary metabolites of the identified fungal species, were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Statistical analysis was performed with principal component analysis (PCA) to characterize the mycotoxin profiles for the twenty fungi, suggesting that these fungi species could be discriminated and divided into three groups as follows. Group I, the fusaric acid producers, were defined into two subgroups, namely subgroup I as producers of fusaric acid and fumonisins, comprising of F. proliferatum, F. verticillioides, F. fujikuroi and F. solani, and subgroup II considered to only produce fusaric acid, including F. temperatum, F. subglutinans, F. musae, F. tricinctum, F. oxysporum, F. equiseti, F. sacchari, F. concentricum, F. andiyazi. Group II, as type A trichothecenes producers, included F. langsethiae, F. sporotrichioides, F. polyphialidicum, while Group III were found to mainly produce type B trichothecenes, comprising of F. culmorum, F. poae, F. meridionale and F. graminearum. A comprehensive picture, which presents the mycotoxin-producing patterns by the selected fungal species in various matrices, is obtained for the first time, and thus from an application point of view, provides key information to explore mycotoxigenic potentials of Fusarium species and forecast the Fusarium infestation/mycotoxins contamination.
外文关键词：Fusarium fungi；mycotoxin profiles；principal component analysis；culture substrates
作者：Shi, W；Tan, YL；Wang, SX；Gardiner, DM；De Saeger, S；Liao, YC；Wang, C；Fan, YY；Wang, ZP；Wu, AB
作者单位：Jiangnan Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：1
点击下载：霉菌毒素谱揭示镰刀菌在各种培养基质中的产毒能力