中文摘要：阻抗分析法是食品安全检测的一种重要工具。本文开发了一种包含金电极芯片和微流细胞新型阻抗式微流控分析系统，该分析系统可灵敏选择性的地检测转基因蛋白Cry1Ab。AntiCry1Ab适体磁珠用于识别转基因蛋白Cry1Ab和组成Cry1Ab适体修饰的磁珠。研究发现，当频率为358.3 Hz时，阻抗信号显示Cry1Ab蛋白在0-0.2 nM的浓度内呈良好的线性关系，检测限为0.015 nM。研究结果表明，阻抗式微流控分析系统提供了另一种可敏感、快速、特异的检测转基因蛋白Cry1Ab蛋白的方法。
外文摘要：Impedimetric analysis method is an important tool for food safety detection. In this work, a novel impedimetric microfluidic analysis system consisted of a printed gold electrode chip and a microfluidic flow cell was developed for sensitive and selective detection of transgenic protein Cry1Ab. AntiCry1Ab aptamer coated magnetic beads were used to recognize transgenic protein Cry1Ab and form Cry1Ab-aptamer modified magnetic beads. After separation, the obtained Cry1Ab-aptamer modified magnetic beads were dissolved in 0.01 M mannitol and followed by injection into the microfluidic flow cell for impedimetric measurement. At the frequency of 358.3 Hz, the impedance signal shows a good linearity with the concentrations of Cry1Ab protein at a range from 0 to 0.2 nM, and the detection limit is 0.015 nM. The results demonstrate that the impedimetric microfluidic analysis system provides an alternative way to enable sensitive, rapid and specific detection of transgenic protein Cry1Ab.
作者：Jin, Shunru; Ye, Zunzhong; Wang, Yixian; 等
作者单位：浙江大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2017
出版刊次：3
点击下载：一种检测转基因蛋白Cry1Ab的新型阻抗式微流控分析系统