中文摘要：分子鉴定为转基因作物的安全评估和后续监测奠定了基础。由于目标的特异性质，以传统聚合酶链反应（PCR）为基础的方法不能全面地检测意外的基因插入，从而导致未知的转基因事件。随着商业化的新型转基因作物（包括新型植物育种技术（NPBT）生成的作物）越来越多，Alternative-Omics方法，特别是下一代测序技术，已被开发用于授权或未经授权的转基因作物的分子鉴定。本文对这些方法进行了综述，解决了他们面临的挑战，并讨论了将分子鉴定用于NPBT生成作物的可行策略，突出了对全球信息共享数据库和具有成本效益的、准确的和综合的分子鉴定方法的需求。
外文摘要：Molecular characterization lays a foundation for safety assessment and subsequent monitoring of genetically modified (GM) crops. Due to the target-specific nature, conventional polymerase chain reaction (PCR)-based methods cannot comprehensively detect unintended gene insertions, let alone unknown GM events. As more and more new developed GM crops including new plant breeding technology (NPBT) generated crops are in the pipeline for commercialization, alternative-omics approaches, particularly next generation sequencing, have been developed for molecular characterization of authorized or unauthorized GM (UGM) crops. This review summarizes first those methods, addresses their challenges, and discusses possible strategies for molecular characterization of engineered crops generated by NPBT, highlighting needs for a global information-sharing database and cost-effective, accurate and comprehensive molecular characterization approaches.
外文关键词：Cisgenesis; Genome editing; Next generation sequencing; T-DNA; Transgenesis
作者：Li, Rong; Quan, Sheng; Yan, Xiaofang; 等
作者单位：上海交通大学
期刊名称：BIOTECHNOLOGY ADVANCES
期刊影响因子：9.848
出版年份：2017
出版刊次：3
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