中文摘要：转基因食品/饲料的监测需要分子技术来提供准确的定量结果。电化学DNA检测技术已在该领域得到了广泛的研究，但大多数报告只显示定性数据，且使用的食品和饲料样品是有限的。本文对电化学多重分析方法在复杂样品DNA量化中的适用性进行评估。本文报告了该方法在转基因食品/饲料调查中的应用，该调查在都和饲料中对抗草甘膦转基因大豆进行了相对定量分析。本文收集了33份大豆样品，其中4份含有未申报的转基因大豆。作者用实时PCR方法来验证这些结果。研究发现，两种方法的检测结果高度相关，这表明本文提出的多重方法适用于食品和饲料监测。
外文摘要：Monitoring of genetically modified organisms in food and feed demands molecular techniques that deliver accurate quantitative results. Electrochemical DNA detection has been widely described in this field, yet most reports convey qualitative data and application in processed food and feed samples is limited. Herein, the applicability of an electrochemical multiplex assay for DNA quantification in complex samples is assessed. The method consists of the simultaneous magnetic entrapment via sandwich hybridisation of two DNA sequences (event-specific and taxon-specific) onto the surface of magnetic microparticles, followed by bienzymatic labelling. As proof-of-concept, we report its application in a transgenic food/feed survey where relative quantification (two-target approach) of Roundup Ready Soybean (RRS) was performed in food and feed. Quantitative coupling to end-point PCR was performed and calibration was achieved from 22 and 243 DNA copies spanning two orders of magnitude for the event and taxon-specific sequences, respectively. We collected a total of 33 soybean-containing samples acquired in local supermarkets, four out of which were found to contain undeclared presence of genetically modified soybean. A real-time PCR method was used to verify these findings. High correlation was found between results, indicating the suitability of the proposed multiplex method for food and feed monitoring.
外文关键词：DNA quantification; Electrochemical multiplex DNA detection; Magnetic microparticles; GMO quantification; Sample analysis
作者：Manzanares-Palenzuela, C. L.; Martin-Clemente, J. P.; Lobo-Castanon, M. J.; 等
作者单位：Univ Complutense Madrid
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：3
点击下载：在转基因食品/饲料定量检测中，利用复合酶标记技术对复杂样品磁捕获DNA序列进行电化学检测