中文摘要：转基因植物的安全性评估是放松管制前所需做的重要工作。转基因植物与其野生非转基因植物的实质等价论证可在不同的分子水平上使用不同的技术来进行。本研究首次对同时表达cry1AcF和cry2Aa基因的转基因木豆进行了综合评价。通过蛋白质组和营养成分分析，没有发现转基因种子成分中有意想不到的影响。研究发现，在各种营养成分分析种，转基因木豆与非转基因木豆没有明显差异。此外，2-DGE蛋白质组分析表明转基因木豆与非转基因木豆的种子蛋白没有大的变化。本文的研究结果还表明，无论是T-DNA的整合或cry基因的表达都会导致植物新毒素或过敏原的形式产生意想不到的效果。
外文摘要：Safety assessment of genetically modified plants is an important aspect prior to deregulation. Demonstration of substantial equivalence of the transgenics compared to their nontransgenic counterparts can be performed using different techniques at various molecular levels. The present study is a first-ever comprehensive evaluation of pigeon pea transgenics harboring two independent cry genes, cry2Aa and cry1AcF. The absence of unintended effects in the transgenic seed components was demonstrated by proteome and nutritional composition profiling. Analysis revealed that no significant differences were found in the various nutritional compositional analyses performed. Additionally, 2-DGE-based proteome analysis of the transgenic and nontransgenic seed protein revealed that there were no major changes in the protein profile, although a minor fold change in the expression of a few proteins was observed. Furthermore, the study also demonstrated that neither the integration of T-DNA nor the expression of the cry genes resulted in the production of unintended effects in the form of new toxins or allergens.
外文关键词：nutritional analysis; pigeon pea; proteome; transgenics
作者：Mishra, Pragya; Singh, Shweta; Rathinam, Maniraj; 等
作者单位：ICAR Res Complex
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：2
点击下载：表达cry1AcF和cry2Aa基因的转基因木豆与其对应非转基因木豆的比较蛋白质组和营养成分分析