中文摘要：一些转基因植物具有抗非生物胁迫的转基因。同时，其他的转基因可能会与非生物胁迫相互作用，从而导致影响植物生理的多效性。因此，生理变化可能会影响产品的安全性。然而，常规风险评估分析没有对不同环境条件下转基因植物的反应进行评估。因此，本文提出了一种抗除草剂玉米的蛋白质组，并在干旱和除草剂胁迫下将它与其近等基因非转基因品种作比较。在不同的干旱程度和除草剂喷洒条件下，作者检测到了转基因和非转基因玉米间的二十个差异蛋白。Pathway富集分析表明，这些蛋白质的大多数参与能量/碳水化合物代谢过程。在激素和相关化合物分析中，不同的玉米品种和环境条件下可检测到不同水平的ABA、CA、JA、MeJA和SA。在Pathway和蛋白质组分析中，环境被认为是变化的主要来源，其次是基因转化因子。然而，在相同的环境条件下，转基因玉米和非转基因玉米中检测到的JA、MeJA、CA水平和11种蛋白的丰度有差异。因此，本文的研究结果可支持分子研究在转基因植物风险评估分析中的应用。
外文摘要：Some genetically modified (GM) plants have transgenes that confer tolerance to abiotic stressors. Meanwhile, other transgenes may interact with abiotic stressors, causing pleiotropic effects that will affect the plant physiology. Thus, physiological alteration might have an impact on the product safety. However, routine risk assessment (RA) analyses do not evaluate the response of GM plants exposed to different environmental conditions. Therefore, we here present a proteome profile of herbicide-tolerant maize, including the levels of phytohormones and related compounds, compared to its near-isogenic non-GM variety under drought and herbicide stresses. Twenty differentially abundant proteins were detected between GM and non-GM hybrids under different water deficiency conditions and herbicide sprays. Pathway enrichment analysis showed that most of these proteins are assigned to energetic/carbohydrate metabolic processes. Among phytohormones and related compounds, different levels of ABA, CA, JA, MeJA and SA were detected in the maize varieties and stress conditions analysed. In pathway and proteome analyses, environment was found to be the major source of variation followed by the genetic transformation factor. Nonetheless, differences were detected in the levels of JA, MeJA and CA and in the abundance of 11 proteins when comparing the GM plant and its non-GM near-isogenic variety under the same environmental conditions. Thus, these findings do support molecular studies in GM plants Risk Assessment analyses.
作者： Benevenuto, Rafael Fonseca; Agapito-Tenfen, Sarah Zanon; Vilperte, Vinicius; 等
作者单位：Univ Fed Santa Catarina
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：2
点击下载：利用蛋白质组学和代谢组学分析来研究转基因玉米对非生物胁迫的分子反应