中文摘要：T1C-19是新开发的、在玉米泛素启动子的控制下表达Cry1C基因的转基因水稻品种，其对水稻鳞翅目害虫有很高的抗性。黑肩绿盲蝽是褐飞虱卵和若虫的主要捕食者，褐飞虱是转基因水稻主要的非靶标刺吸式害虫。黑肩绿盲蝽可能会接触Cry1C蛋白，因此表达cry1c基因的转基因水稻对黑肩绿盲蝽的安全性评估应在T1C-19商业化之前进行。本文测试了高剂量的Cry1C对黑肩绿盲蝽的直接毒性、T1C-19对黑肩绿盲蝽（通过捕食褐飞虱）生命参数的影响、T1C-19对稻田中黑肩绿盲蝽的种群密度和动态的影响。研究发现，将黑肩绿盲蝽直接暴露于高剂量的Cry1C蛋白或通过猎物暴露于真实蛋白剂量，对黑肩绿盲蝽的发育、存活、雌性比和体重没有不利影响。黑肩绿盲蝽在T1C-19和非转基因水稻田地中的种群密度和动态没有显著差异。研究结果表明，表达cry1c基因的转基因水稻对黑肩绿盲蝽没有不利影响。这是研究表达cry1c基因的转基因水稻对黑肩绿盲蝽影响的首次报告。
外文摘要：T1C-19, a newly developed transgenic cry1C rice line, expresses cry1C under the control of the maize ubiquitin promoter, and is highly resistant to lepidopteran pests of rice. Cyrtorhinus lividipennis is the major predator of the eggs and young nymphs of Nilaparvata lugens, which is the main non-target sap-sucking insect pest of Bt rice. C. lividipennis may be exposed to Cry1C protein, thus biosafety evaluations of transgenic cry1C rice on C. lividipennis should be conducted before the commercialization of T1C-19. In the current study, we tested the direct toxicity of elevated doses of Cry1C to C. lividipennis, effects of T1C-19 on the life-table parameters of C. lividipennis via preying planthoppers, and effects of T1C-19 on the population density and dynamics in rice fields. No detrimental effects on development, survival, female ratio and body weight of C. lividipennis were caused by direct exposure to elevated doses of the Cry1C protein or prey-mediated exposure to realistic doses of the protein. The population density and dynamics did not significantly differ between C. lividipennis in T1C-19 and non-transgenic rice fields. Thus, transgenic cry1C rice had no negative effects on C. lividipennis. This is the first report of the effects of transgenic cry1C rice on C. lividipennis.
作者：Han, Yu; Ma, Fugang; Nawaz, Muhammad; 等
作者单位：Huazhong Agr Univ
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2017
出版刊次：2
点击下载：表达cry1c基因的转基因水稻对褐飞虱的主要捕食者黑肩绿盲蝽影响的分层评价研究