中文摘要：本研究建立了一个检测玉米褪绿斑驳病毒的一步逆转录环介导等温扩增技术。该技术基于外壳蛋白基因序列设计了一套病毒特异性引物，在60分钟内即可完成检测，63°C等温条件下，该技术的灵敏度比RT-PCR技术高出十倍，且与其他病毒性病原体没有交叉反应，检测结果可以在染色封闭管中通过SYBR屏I观察到，并且可促进MCMV检测的高通量应用。该方法已经通过现场采集测试进行了验证，表明MCMV RT-LAMP技术是一种快速、高效、灵敏的方法，可用于MCMV感染的常规筛选。
外文摘要：Maize chlorotic mottle virus (MCMV) is spreading in many regionsworldwide, causing maize lethal necrosis when co-infected with apotyvirid. In this study, one-step reverse transcription loop-mediatedisothermal amplification (RI-LAMP) assay was developed to detect MCMV inmaize. A set of four specific primers was designed based on theconserved coat protein gene sequences of MCMV. The RT-LAMP could becompleted within 60 min under isothermal condition at 63 degrees C. Thesensitivity test showed that the RT-LAMP was about 10-fold moresensitive than RT-PCR and no cross-reactivity was detected with otherviral pathogens infecting maize in China. Moreover, the results of RT-LAMP could be visually inspected by SYBR Green I staining in aclosed-tube, facilitating high-throughput application of MCMV detection.This method was further verified by testing field-collected samples.These results suggested that the developed MCMV RT-LAMP technique is arapid, efficient and sensitive method which could be used as a routinescreen for MCMV infection. (C) 2016 Elsevier B.V. All rights reserved.
 
外文关键词：Maize chloro tic mottle virus (MCMV), Loop-mediated isothermal amplification (LAMP), Maize, Detection
作者：Chen, Ling; Jiao, Zhiyuan; Liu, Dongmei; Liu, Xingliang; Xia, Zihao; Deng, Congliang; Zhou, Tao; Fan, Zaifeng
作者单位：China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China.
期刊名称：JOURNAL OF VIROLOGICAL METHODS
期刊影响因子：1.508
出版年份：2017
出版刊次：2
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