中文摘要：本研究制备了一种应用酶级联放大策略的新的比色传感平台，与MnO2纳米片的酶控溶解相结合用于定量检测小分子霉菌毒素（本研究采用黄曲霉毒素B1，AFB1）。研究发现基于MnO2-TMB的免疫分析系统的精密度和特异性均在可接受范围内。此外，用花生样品进一步验证该方法的精确度，检测结果与用商业化的AFB1 ELISA试剂盒得到的结果高度一致。
外文摘要：A new colorimetric immunosensing platform accompanying enzyme cascade amplification strategy was fabricated for quantitative screening of small-molecular mycotoxins (aflatoxin B1, AFB1 used in this case) coupling with enzyme-controlled dissolution of MnO2 nanoflakes. The visual colored assay was executed by high-efficient MnO2-3,3',5,5'-tetramethylbenzidine (TMB) system (blue). In the presence of ascorbic acid, MnO2 nanoflakes were dissolved into Mn2+ ions, thus resulting in a perceptible color change from blue to colorless. The reaction could be weakened through ascorbate oxidase to catalyze ascorbic acid into dehydroascorbic acid, which indirectly depended on the concentration of ascorbate oxidase. By using ascorbate oxidase/ anti-AFB1 antibody-labeled gold nanoparticles, a novel competitive-type colorimetric enzyme immunoassay was developed for detection of AFB1 on AFB1-bovine serum albumin (BSA)-conjugated magnetic beads. Upon addition of target AFB1, the analyte competed with the conjugated AFB1-BSA on the magnetic beads for the labeled anti-AFB1 antibody on the gold nanoparticles. Under optimal conditions, the absorbance decreased with increasing target AFB1 within the dynamic range of 0.05-150ngmL-1 with a detection limit of 6.5pgmL-1 at the 3Sblank level. The precision and specificity of the MnO2-TMB-based immunosensing system were acceptable. In addition, method accuracy was further validated for monitoring spiked peanut samples, giving results matched well with those obtained from commercialized AFB1 ELISA kit.
外文关键词：MnO2 nanoflakes；Colorimetric immunoassay；Aflatoxin B-1；Enzyme cascade amplification
作者：Lai, WQ；Wei, QH；Xu, MD；Zhuang, JY；Tang, DP
作者单位：Fuzhou Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2015
出版刊次：12
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