中文摘要：赭曲霉毒素A（OTA）是一种霉菌毒素，由某些真菌如赭曲霉、黑曲霉、炭黑曲霉和鲜绿青霉产生，是全球许多食品的自然污染物。摄入OTA会对人和动物产生毒害，如肾和肝毒性。虽然食品污染的相关资料很多，但巴西缺少有关OTA人体暴露的资料。因此，本研究旨在确定人体中的OTA水平，并识别可能与肝和肾损伤相关的生物标志物。本文首次研究了巴西人体受OTA污染的情况，由受试人群血浆中OTA的水平推断，每周摄入量低于欧盟委员会容许的食物链中污染物的每周耐受摄入量。但是，还需要在更大的区域范围和在不同的季节进行纵向的延伸研究
外文摘要：Ochratoxin A (OTA), a mycotoxin produced by some fungi like Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius and Penicillium viridicatum, is a natural contaminant of many foods worldwide. The intake of OTA is associated with deleterious effects to humans and animals, such as nephro-and hepatotoxicity. Although there are some data about food contamination, there is lack of data about human exposure to OTA in Brazil. Therefore, current research aimed to determine the level of human exposure to OTA and, additionally, identify possible associations with biomarkers of liver and kidney damage. OTA levels were evaluated in plasma samples from 149 individuals living in the state of Parana, Brazil, by indirect competitive ELISA using monoclonal antibody anti-OTA (cell line OTA. 7). Plasma levels of OTA, alanine aminotransferase, aspartate aminotransferase, urea and creatinine were submitted to Pearson's correlation test. It was possible to measure OTA levels in 54.4% of the samples (mean 734 +/- 296 pg/ml; maximum 1,585 pg/ml), with an estimated daily intake of 983-1,445 pg/kg body weight. There was no correlation between OTA plasma levels and biochemical parameters, possibly due to the low level of contamination. This is one of the first studies concerning the contamination of humans by OTA in Brazil and we conclude that the plasma levels of the evaluated population indicate an estimated weekly intake below the tolerable weekly intake derived by the EFSA Panel on Contaminants in the Food Chain. Nevertheless, additional longitudinal studies with greater regional coverage and at different seasonal periods are necessary.
外文关键词：blood；hepatotoxicity；ic-ELISA；mycotoxins；nephrotoxicity
作者：Rigobello, FF；Silva, PLAE；Yamashita, CRT；Lenhard-Vidal, A；Ishikawa, AT；Kawamura, O；Hirooka, EY；Itano, EN
作者单位：Univ Estadual Londrina
期刊名称：World Mycotoxin Journal
期刊影响因子：2.091
出版年份：2016
出版刊次：4
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