中文摘要：探针材料对改善侧流免疫层析试纸条(ICTS)的检测效率和灵敏度有着非常重要的作用。与常规ICTS分析通常使用单组分、纯金纳米粒子作探针不同，本研究成功开发了采用双金属、中空的Au-Ag纳米粒子(NPs)标记的ICTS用于检测瘦肉精(CLE)。结果发现，中空Au-Ag NPs探针的灵敏度在短时间（15分钟）内达到了2 ppb，优于用Au NPs的常规试纸的检测性能。表明中空Au-Ag NPs标记的试纸是用于快速检测食品样品中CLE的理想选择。
外文摘要：The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.
作者：Wang, JY；Zhang, L；Huang, YJ；Dandapat, A；Dai, LW；Zhang, GG；Lu, XF；Zhang, JW；Lai, WH；Chen, T
作者单位：Nanchang Univ
期刊名称：Scientific Reports
期刊影响因子：5.228
出版年份：2017
出版刊次：1
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