中文摘要：本研究首次提出一种用铁（II，III）氧化物(Fe3O4)/ 多壁碳纳米管(MWCNT)复合材料作吸附剂的可靠的固相提取(SPE)法用于纯化和富集丹参中的玉米赤霉烯酮及四种A型单端孢霉烯毒素（T-2毒素(T-2), HT-2毒素(HT-2), 新茄镰孢菌醇 (NEO)和蛇形菌素 (DAS)）。在最佳样品预处理条件下，本方法结合超高效液相色谱-串联质谱，得到了良好的线性(R2≥0.991)、较高的灵敏度（定量限为1.20-4.80μg/kg）、较好的回收率 (73.7-91.9%)及较高的准确性(RSD, 2.1-13.3%)。经验证后，在真实的丹参样品中进一步证实了该方法的适用性。
外文摘要：For the first time, a reliable solid-phase extraction (SPE) procedure using iron (II, III) oxide (Fe3O4)/multi-walled carbon nanotube (MWCNT) composite as sorbents was proposed for purification and enrichment of zearalenone (ZEA) and four type A trichothecenes (T-2 toxin (T-2), HT-2 toxin (HT-2), neosolaniol (NEO) and diacetoxyscirpenol (DAS)) in Salviae miltiorrhiza Radix et Rhizoma (Danshen). The Fe3O4/MWCNT composite was synthesized by assembling Fe3O4 with MWCNT by sonication through an "aggregation wrap" mechanism and several key parameters affecting the performance of SPE procedure such as the composition of sample loading solutions, washing and elution solvents were thoroughly investigated. After optimization, 2% acetonitrile aqueous solution as the loading solution, 5% methanol aqueous solution as the washing solution and acetone containing 0.5% formic acid as the elution solvent presented an excellent purification efficiency for the five targets in Danshen. Under the optimal sample pretreatment conditions followed by analysis with ultra-high performance liquid chromatography-tandem mass spectrometry, satisfactory linearity (R2≥0.991), high sensitivity (limit of quantification in the range of 1.20-4.80μgkg-1), good recovery (73.7-91.9%) and acceptable precision (RSD, 2.1-13.3%) were obtained. The applicability of the validated method was further verified in real Salviae miltiorrhiza Radix et Rhizoma samples.
外文关键词：Salviae miltiorrhiza Radix et Rhizoma；Zearalenone；Type A trichothecenes；Solid-phase extraction；Iron (II,III) oxide/multi-walled carbon nanotube composite；Ultra-high performance liquid；chromatography-tandem mass spectrometry
作者：Jiang, KQ；Huang, P；Luan, LJ；Fan, K；Guo, WB；Zhao, ZH；Wu, YJ；Han, Z
作者单位：Zhejiang Univ
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：1
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