中文摘要：本研究采用一种QuEChERS（快速、方便、廉价、高效、稳定而安全）的提取流程开发了一种简便有效的检测植物油中多种霉菌毒素的方法。用高效液相色谱-串联质谱(HPLC-MS/MS) 定量鉴定16种化学多样性霉菌毒素。本方法具有高灵敏度、准确度和选择性的优点，并可用于实际样品中目标毒素的筛选。
外文摘要：A simple and efficient method for determining multiple mycotoxins was developed using a QuEChERS (quick, easy, cheap, effective, rugged and safe)-based extraction procedure in vegetable oils. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for the quantification and confirmation of 16 chemically diversified mycotoxins. Different extraction procedures were studied and optimised by spiking 16 analytes into blank matrix, and the extraction with 85% MeCN solution and C18 as cleaning sorbent allowed an efficient recovery of 72.8–105.8% with RSDs less than 7%. The limit of detection (LOD) ranged from 0.04 to 2.9 ng g–1. The developed method was finally applied to screen mycotoxins in 62 vegetable oil samples. Zearalenone (ZEN), aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and α-zearalenol (α-ZOL) were detected, with maximum concentrations of 0.59 (AFG1)–42.5 (ZEN) ng g–1. The method developed has the advantages of high sensitivity, accuracy and selectivity, and it can be applied to the target screening of mycotoxins in real samples.
外文关键词：Mycotoxins；vegetable oil；QuEChERS；HPLC-MS；MS
作者：Zhao, HX；Chen, XY；Shen, C；Qu, BC
作者单位：Dalian Univ Technol
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2017
出版刊次：2
点击下载：用QuEChERS法结合高效液相色谱-串联质谱检测植物油中的16种霉菌毒素