中文摘要：本研究旨在通过组织学病变检测、细胞周期时相分布和细胞凋亡，对比评估受黄曲霉毒素污染的玉米对小鸡胸腺和法氏囊(BF)的作用。研究表明，组织学病变的严重性可能与AFB暴露下两种中枢免疫器官的敏感性不同相关；不同的细胞周期阻滞时相说明可能涉及到胸腺和BF的不同机理，对此还需作深入研究.
外文摘要：This study aimed to evaluate the comparative effects of aflatoxin-contaminated corn on the thymus and bursa of Fabricius (BF) in chickens by detecting histopathological lesions, cell cycle phase distribution and apoptosis. A total of 900 COBB500 male broilers were randomly allocated into five groups. The experiment lasted for six weeks and the five dietary treatments consisted of uncontaminated corn (control), 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups. The gross changes showed the decreased size of the thymus and BF, as well as the pale color of the BF in the broilers after aflatoxin contaminated diet exposure. There were more nuclear debris in the thymus and BF of birds in the 50%, 75%, and 100% contaminated corn groups, but the pathological impairments of the BF were more obvious than those of the thymus, which showed as more obvious lymphocyte depletion and the proliferation of reticulocytes and fibroblasts. At 21 days of age, the percentage of thymocytes and BF cells in the G2M phase was increased in a dose-dependent manner in the four AFB-contaminated corn groups. However, at 42 days of age, dietary AFB₁ induced cell cycle perturbation at the G0G1 phase in thymocytes, but at the G2M phase in BF cells. The increased percentage of apoptotic cells in the thymus and BF were similarly observed in the AFB groups. According to these results, the severity of histopathological lesions may be correlated with the different sensitivity of the two central immune organs when exposed to AFB; different arrested cell cycle phases suggest that different mechanisms may be involved in the lesions of the thymus and BF, which need to be further researched.
外文关键词：aflatoxin；comparative lesion；thymus；bursa of Fabricius
作者：Peng, X；Bai, SP；Ding, XM；Zhang, KY
作者单位：Sichuan Agr Univ
期刊名称：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
期刊影响因子：2.035
出版年份：2017
出版刊次：1
点击下载：黄曲霉毒素污染的玉米引起的肉鸡胸腺及法氏囊的病理损害、细胞周期阻滞及凋亡的研究