中文摘要：脱氧雪腐镰刀菌烯醇（DON）及其隐蔽形式（3-，和5-乙酰DON，DON-3-葡萄糖苷）通常用色谱法进行分析。诚然，结合适当的提取和洗涤，LC-MS代表着检测多种霉菌毒素的最佳方法。另一方面，基于免疫化学的方法虽然到目前为止无法对单一成份进行检测，却可能检测到一系列结构相关的化合物。但是，ELISA法通常由于霉菌毒素的隐蔽形式以及基质成份可能与(为母体毒素设计的)抗体发生交叉反应而导致毒素含量的明显高估。迄今，已有文献报道了商业DON检测ELISA试剂盒关于可能交叉反应的几个数据。这些数据通常是在缓冲溶液或基质匹配溶液中获得的，但从未报道过一组自然产生的样品的比较。本研究评估了一种商业DON检测ELISA试剂盒用于自然产生的软质小麦（n=15）和玉米(n = 15)的准确性，考虑了基质效应。根据我们的数据，ELISA是一个有前途的用于准确评估软质小麦及玉米样品中的DON及其隐蔽形式，表示为总DON、DON-3Glc和3Ac-DON的群组检测工具.
外文摘要：Deoxynivalenol (DON) and its modified forms (3-, and 15-acetyl-DON, DON-3-glucoside) are commonly analysed by chromatographic methods. Indeed, coupled with proper extraction and clean-up, LC-MS represents the best approach for multi-mycotoxin measurements. On the other hand, immunochemistry-based methods are possibly able to detect a family of structurally related compounds, although the determination of single contributions is not possible so far. However, ELISA methods often lead to an apparent overestimation of the mycotoxins content because modified forms and matrix components can potentially cross-react with the antibodies (designed for the parent toxin). Several data about the possible cross-reactivity of commercial DON-detecting ELISA kit are reported in the literature so far. Data are commonly obtained in buffer solutions or in matrix-matched solutions, but comparison of a set of naturally incurred samples has never been reported. In the present work the accuracy of a commercial DON-detecting ELISA kit was evaluated on naturally incurred soft wheat (n = 15) and maize (n = 15), taking into account the matrix effect. Recovery was calculated considering the DON concentration found by LC-MS/MS and the total DON concentration, expressed as the sum of DON and its modified forms found by LC-MS/MS. The obtained data clearly show that, when 3-modified forms of DON occur in the sample, the ELISA kit does actually detect them, thus returning an apparent overestimation if only DON content is considered. When the ELISA recovery is calculated on the total DON content, the accuracy of the analysis increases and the variability decreases. According to our data, the ELISA kit seems to be a promising group detection tool for the accurate evaluation of DON and its modified forms, expressed as sum of DON, DON-3Glc and 3Ac-DON, for soft wheat and maize samples.
外文关键词：modified mycotoxins；rapid methods；grains；Fusarium
作者：Righetti, L；Galaverna, G；Dall'Asta, C
作者单位：Univ Parma
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2017
出版刊次：2
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