中文摘要：量子点和胶体金纳米颗粒都是谷物基质中霉菌毒素脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和T2 / HT2毒素的多元横向流动免疫标签，并且两者基于相同的免疫剂，因此可对两种标签的特性进行客观的比较分析。通过比较我们发现，在没有特殊装置的情况下，两种多元横向流动免疫标签都可对小麦中霉菌毒素脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和T2 / HT2毒素进行快速、高效、方便的定性检测，但是基于量子点标签的多元横向流动免疫检测消耗的免疫试剂较少、敏感性更强、经济效益更高且结果更容易解释。
外文摘要：Quantum dots (QDs) and colloidal gold nanoparticles (CG) were evaluated as labels for multiplex lateral flow immunoassay (LFIA) for determination of mycotoxins deoxynivalenol (DON), zearalenone (ZEN) and T2/HT2-toxin (T2/HT2) in cereal matrices. Both developed assays were based on the same immunore-agents (except for the labels), therefore their analytical characteristics could be objectively compared. For both LFIAs antigens (DON-ovalbumin (OVA), ZEN-OVA and T2-OVA) and rabbit anti-mouse immunoglobulin were immobilized on a nitrocellulose membrane as three test lines and one control line, respectively. Depending on the LFIA, monoclonal antibodies (mAb) against DON, ZEN and T2 were conjugated with CdSeS/ZnS QDs or CG. T2 and HT2 were detected by one test line (T2-OVA) with an anti T2 mAb which showed 110% cross-reactivity with HT2. Both tests were developed in accordance with the legal limits and were developed in such a way that they had the same cut-off limits of 1000 mu g kg(-1), 80 mu g kg(-1) and 80 mu g kg (-1) for DON, ZEN and T2/HT2, respectively in order to allow a correct comparison. Applicability of these assays was demonstrated by analysis of naturally contaminated wheat samples. The results demonstrate that both the LFIAs can be used as rapid, cost-effective and convenient qualitative tool for on-site screening for simultaneous detection of DON, ZEN and HT2/T2 in wheat without special instrumentation. However, the QD-based LFIA consumed less immunoreagents and was more sensitive and economically beneficial. In addition, the results were easier to interpret, resulting in a lower false negative rate (<5%) which was in good agreement with Commission Decision-2002/657/EC regarding the performance of analytical methods intended for screening purposes. (C) 2016 Elsevier B.V. All rights reserved.
外文关键词：Quantum dots, Colloid gold nanoparticle, Bioconjugation, Multiplex lateral flow immunoassay, Mycotoxin
作者：Foubert, Astrid; Beloglazova, Natalia V.; De Saeger, Sarah
作者单位：Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, Ghent, Belgium
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：4.712
出版年份：2017
出版刊次：2
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