中文摘要：黄瓜绿斑驳花叶病毒会导致西瓜和黄瓜严重花叶症状，并通过感染黄瓜种子、叶子和土壤进行传播。为了有效地检测黄瓜绿斑驳花叶病毒，防止其引起的感染在田间传播，本文以未改进金纳米粒子为探针构建了一个简单且敏感的无标记比色检测方法，通过NaCI诱导后金纳米粒子由红变蓝的颜色变化来识别目标病毒的存在。通过这种方法，不需要任何复杂昂贵的仪器和生化试剂，使得黄瓜绿斑驳花叶病毒RNA肉眼视觉检测低至30 pg/μL，特异性为100%且重现性良好。结果表明，该方法是一种高种属特异性、简单、低成本、直观方便地检测植物组织中黄瓜绿斑驳花叶病毒的方法，对中小型企业以及出入境检验检疫局对黄瓜种子或植物组织进行视觉检测方面具有较大的应用潜力。
外文摘要：Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber
seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCI induction. Normally, species-specific probes attach to the surface of AuNP5 and thereby increasing their resistance to NaCI-induced aggregation. The concentration of sodium, probes in the reaction system and evaluation of specificity and sensitivity of a novel assay, visual detection of Cucumber green mottle mosaic virus using unmodified AuNP5 has been carried out with simple preparation of samples in our study. Through this assay, as low as 30 pg/μL of CGMMV RNA was thus detected visually, by the naked eye, without the need for any sophisticated, expensive instrumentation and biochemical reagents. The specificity was 100% and exhibited good reproducibility in our assays. The results note that this assay is highly species-specific, simple, low-cost, and visual for easy detection of CGMMV in plant tissues. Therefore, visual assay is a potentially useful tool for middle or small-scales corporations and entry-exit inspection and quarantine bureau to detect CGMMV in cucumber seeds or plant tissues. (C) 2017 Elsevier B.V. All rights reserved.
 
外文关键词：Visual detection, Cucumber green mottle mosaic virus, Gold nanoparticles
作者：Wang, Lin; Liu, Zhanmin; Xia, Xueying; Yang, Cuiyun; Huang, Junyi; Wan,Sibao
作者单位：Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China.
期刊名称：JOURNAL OF VIROLOGICAL METHODS
期刊影响因子：1.508
出版年份：2017
出版刊次：3
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