中文摘要：在越南，每天都有大量有毒物质，包括工农业活动、食品生产及健康服务机构产生的致癌及前致癌物质释放到环境中。本研究报道了通过共转化两个质粒的新型酵母生物传感系统检测遗传致癌物和前致癌物的进展。研究发现结合液体处理机器人，该平台能以完全自动化和连续的方式，快速、经济而高通量地筛选多种目标物，而无需用户交互。
外文摘要：In Vietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. In the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. One plasmid is carrying human CPR and CYP (CYP3A4, CYP2B6, or CYP2D6) genes, while the other contains the RAD54-GFP reporter construct. The three resulting coexpression systems bearing both CPR-CYP and RAD54-GFP expression cassettes were designated as CYP3A4/CYP2B6/CYP2D6 + RAD54 systems, respectively and used to detect and evaluate the genotoxic potential of carcinogens and procarcinogens by selective activation and induction of both CPR-CYP and RAD54-GFP expression cassettes in response to DNA damage. Procarcinogens were shown to be predominantly, moderately or not bioactivated by one of the CYP enzymes and thus selectively detected by the specific coexpression system. Aflatoxin B1 and benzo(a)pyrene were predominantly detected by the CYP3A4 + RAD54 system, while N-nitrosodimethylamine only moderately activated the CYP2B6 + RAD54 reporter system and none of them was identified by the CYP2D6 + RAD54 system. In contrast, the genotoxic carcinogen, methyl methanesulfonate, was detected by all systems. Our yeast-reporter system can be performed in 384-well microplates to provide efficient genotoxicity testing to identify various carcinogenic compounds and reduce chemical consumption to about 53% as compared with existing 96-well genotoxicity bioassays. In association with a liquid handling robot, this platform enables rapid, cost-effective, and high-throughput screening of numerous analytes in a fully automated and continuous manner without the need for user interaction.
作者：Bui, VN；Nguyen, TTH；Mai, CT；Bettarel, Y；Hoang, TY；Trinh, TTL；Truong, NH；Chu, HH；Nguyen, VTT；Nguyen, HD；Wolfl, S
作者单位：VAST
期刊名称：PLoS One
期刊影响因子：3.057
出版年份：2016
出版刊次：12
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