中文摘要：本研究旨在从肉鸡环境如饲料、粪便和肠中分离和表征天然酵母菌株，并评估它们结合黄曲霉毒素B1（AFB1）的能力。结果发现本研究测试的酵母菌株构成了潜在的AFB1吸附剂而且具有从禽类环境中获取的优势。本研究为利用从肉鸡环境中获得天然酵母控制禽类生产中慢性黄曲霉毒素中毒做出了贡献。
外文摘要：AIMS: isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1 ). METHODS AND RESULTS: A total of 9 yeast strains were isolated: 3 from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), 2 from gut identified as Candida tropicalis and 4 from faeces identified as C. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out the adsorptions studies, one strain from each genus and each origin was selected as follows: C. lusitaniae and P. kudriavzevii from feedstuff, C. lusitaniae and Cyberlindnera fabianii from feaces and C. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cell ml-1 and 100 ng ml-1 of AFB1 , respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherms studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the complex AFB1 yeast demonstrated the irreversibility of the binding process. CONCLUSION: Yeast strains tested in the present work constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. SIGNIFICANCE AND IMPACT OF THE STUDY: This work makes a contribution on using of native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.
外文关键词：adsorption；aflatoxin B-1；broilers' ecosystem；native；yeast
作者：Magnoli, AP；Rodriguez, MC；Poloni, VL；Rojo, MC；Combina, M；Chiacchiera, SM；Dalcero, AM；Cavaglieri, LR
作者单位：Univ Nacl Rio Cuarto
期刊名称：JOURNAL OF APPLIED MICROBIOLOGY
期刊影响因子：2.156
出版年份：2016
出版刊次：6
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