中文摘要：为了保护人们免受黄曲霉毒素M1(AFM1)已证实的毒性和致癌性的侵害，需要连续控制牛奶中存在的AFM1。近年来，越来越需要开发可靠的牛奶样品中低浓度AFM1的灵敏、准确、简便而快速的检测方法。为此，优化并验证了酶联免疫吸附测定(ELISA)、高效液相色谱荧光检测器 (HPLC-FLD) 及高效液相色谱-串联质谱(HPLC-MS/MS)，用它们分析自然污染的牛奶样品中的AFM1，并评估了三种不同方法的结果之间的一致性。研究发现三种方法都能满足官方控制目的的要求，就作者所知，本研究报道的研究和比较用ELISA、HPLC-FLD和HPLC-MS/MS 三种方法检测自然污染的牛奶样品中的AFM1尚属首次。
外文摘要：Presence of aflatoxin M1 (AFM1) in milk should be continuously controlled in order to protect the population from risks associated with its proven toxicity and carcinogenicity. During recent years, there has been an increase in demand for development of sensitive, accurate, simple and fast method which is reliable for detection of AFM1 at low concentrations found in milk samples. For that purpose, enzyme linked immunosorbent asssay (ELISA), high performance liquid chromatography with fluorescence detector (HPLC-FLD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were optimized and validated in order to apply them for AFM1 analysis in naturally contaminated milk samples, and to assess the closeness of agreement between results of three different methods. The obtained validation parameters indicate that all three methods are suitable for determination of AFM1 in milk samples. The statistical analysis of variance between the methods and the obtained correlation coefficients indicate that there is a strong correlation between methods. All three methods are satisfactory in meeting the requirements for official control purposes. To the best of author's knowledge, this study represents the first report of an investigation and comparison of ELISA, HPLC-FLD and HPLC-MS/MS methods for determination of AFM1 in naturally contaminated milk samples.
外文关键词：Milk；aflatoxin M1；ELISA；HPLC-FLD；HPLC-MS/MS
作者：Kos, J；Hajnal, EJ；Jajic, I；Krstovic, S；Mastilovic, J；Saric, B；Jovanov, P
作者单位：Univ Novi Sad
期刊名称：ACTA CHIMICA SLOVENICA
期刊影响因子：1.167
出版年份：2016
出版刊次：4
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