中文摘要：根据欧盟和瑞士食品法规规定，只有转基因植物的授权性状才允许进口和销售给消费者。为了控制零售商处玉米产品的进口，我们需要高效可靠的检测和定量方法。目前在欧盟有超过25个的转基因玉米性状被授权并允许出售。为了在销售中确保正确地标记和排除未经授权的转基因性状，本文开发了五重实时聚合酶链反应系统，并对该系统进行了验证。研究结果表明，五重实时PCR系统可以可靠、成本和时间有效的方式来量化和检测这20个转基因玉米的性状。
外文摘要：According to the EU and Swiss food legislation, only authorised traits of transgene plants are allowed to be imported and sold to the consumer. In order to control imports of maize products from retailers, efficient and reliable methods for the detection and quantification are a prerequisite. Actually, in the EU, more than 25 gm Maize traits were authorised and are allowed to be sold. To ensure correct labelling and exclude unauthorised gm traits from sale, five multiplex real-time polymerase chain reaction systems were developed and validated. These 20 systems for the most prevalent maize gm traits allow relative multiplex quantification and/or delta-delta Ct method quantification in routine in a reliable, cost- and time-efficient way.
外文关键词：gm Maize; Multiplex; Real-time quantitative PCR; Validation
作者：Koppel, Rene; Ganeshan, Arthika; van Velsen, Franziska; 等
作者单位：Official Food Control Author Canton Zurich
期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY
期刊影响因子：1.433
出版年份：2017
出版刊次：2
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