中文摘要：近年来，转基因技术在许多领域得到了广泛的应用。随着转基因产品的增加，转基因产品的安全性受到了人们的关注。为了预防奶牛乳腺炎，本文研发出了转人β防御素-3基因牛，它可以防御由细菌引起的乳腺炎。因此，转基因牛的牛奶中包含HBD3。本文的研究目的是分析HBD3牛奶的营养成分和对大鼠进行90天的喂养试验。大鼠被随机分为5组，五组分别喂养含有10%HBD3牛奶的AIN93G饮食、含有30%%HBD3牛奶的AIN93G饮食、含有10%正常牛奶的AIN93G饮食、含有30%%正常牛奶的AIN93G饮食、AIN93G饮食。研究发现，HBD3牛奶与常规牛奶的营养成分无显著差异。此外，饮食中含有HBD3牛奶的大鼠在体重、食物消耗量、血生化指标、脏器重量和组织病理学检查等性状上均正常。
外文摘要：In recent years, transgenic technology has been widely applied in many fields. There is concern about the safety of genetically modified (GM) products with the increased prevalence of GM products. In order to prevent mastitis in dairy cows, our group produced transgenic cattle expressing human beta-defensin-3 (HBD3) in their mammary glands, which confers resistance to the bacteria that cause mastitis. The milk derived from these transgenic cattle thus contained HBD3. The objective of the present study was to analyze the nutritional composition of HBD3 milk and conduct a 90-day feeding study on rats. Rats were divided into 5 groups which consumed either an AIN93G diet (growth purified diet for rodents recommended by the American Institute of Nutrition) with the addition of 10% or 30% HBD3 milk, an AIN936, diet with the addition of 10% or 30% conventional milk, or an AIN93G diet alone. The results showed that there was no difference in the nutritional composition of HBD3 and conventional milk. Furthermore, body weight, food consumption, blood biochemistry, relative organ weight, and histopathology were normal in those rats that consumed diets containing HBD3. No adverse effects were observed between groups that could be attributed to varying diets or gender.
外文关键词：Genetically modified milk; HBD3; Safety assessment; 90-Day feeding study
作者：Chen, Xin; Gao, Ming-Qing; Liang, Dong; 等
作者单位：Northwest A&F Univ
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.584
出版年份：2017
出版刊次：2
点击下载：通过为期90天的喂养试验来进行转人β防御素-3基因牛奶对大鼠的安全性评估