中文摘要：为了评估转基因杨树林对生态系统的可能影响，本文分析了成熟雄性转Bt基因杨树（表达苏云金芽孢杆菌毒素基因）与其存活种子间的基因流动的频率和距离。作者将Bt基因杨树种子以15% 0 m到0.02% 50m的频率种植在转Bt基因杨树林周围。研究结果表明，源自转Bt基因杨树林的基因流动可以在自然条件下通过花粉或种子等非常低的水平下发生。本文研究为转基因杨树林转基因流动的研究提供了第一手资料，并为转基因杨树林的风险评价提供了一些指导。
外文摘要：To assess the possible impact of transgenic poplar plantations on the ecosystem, we analyzed the frequency and distance of gene flow from a mature male transgenic Populus nigra plantation carrying the Bacillus thuringiensis toxin gene (Bt poplar) and the survival of Bt poplar seeds. The resultant Bt poplar seeds occurred at a frequency of similar to 0.15% at 0 m to similar to 0.02% at 500 m from the Bt poplar plantation. The germination of Bt poplar seeds diminished within three weeks in the field (germination rate from 68% to 0%) compared to 48% after three weeks of storage at 4 degrees C. The survival rate of seedlings in the field was 0% without any treatment but increased to 1.7% under the addition of four treatments (cleaning and trimming, watering, weeding, and covering with plastic film to maintain moisture) after being seeded in the field for eight weeks. The results of this study indicate that gene flow originating from the Bt poplar plantation occurred at an extremely low level through pollen or seeds under natural conditions. This study provides first-hand field data on the extent of transgene flow in poplar plantations and offers guidance for the risk assessment of transgenic poplar plantations.
作者：Hu, Jianjun; Zhang, Jin; Chen, Xingling; 等
作者单位：Chinese Acad Forestry
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：1
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