中文摘要：蚜虫是数量最多的刺吸式害虫，它每年在全球范围内可导致严重的农作物产量损失。而大量使用杀虫剂来对付蚜虫会对环境造成严重的破坏，并危害人类健康。本文用CaMV 35S和ST-LS1启动子生成了六个转基因品种，从而研发出了表达雪花莲凝集素（GNA）基因的转基因马铃薯。实时定量聚合酶链反应（qRT-PCR）分析表明，在aMV 35S启动子的控制下，GNA基因在转基因马铃薯的叶、茎、根中表达；而在ST-LS1启动子的控制下，GNA基因只在转基因马铃薯的叶、茎中表达。接种5天后，转基因马铃薯中蚜虫的死亡率为20%-53.3%；接种15天后，单株转基因马铃薯中的蚜虫量为17.0 +/- 1.43（ST2）和36.6 + 0.99（35S3），是非转基因马铃薯中的蚜虫量的24.9%-53.5%。研究结果表明，转基因马铃薯中GNA基因的表达可防御蚜虫攻击，这将在未来代替农药的使用。
外文摘要：Aphids, the largest group of sap-sucking pests, cause significant yield losses in agricultural crops worldwide every year. The massive use of pesticides to combat this pest causes severe damage to the environment, putting in risk the human health. In this study, transgenic potato plants expressing Galanthus nivalis agglutinin (GNA) gene were developed using CaMV 35S and ST-LS1 promoters generating six transgenic lines (35S1-35S3 and ST1-ST3 corresponding to the first and second promoter, respectively). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the GNA gene was expressed in leaves, stems and roots of transgenic plants under the control of the CaMV 35S promoter, while it was only expressed in leaves and stems under the control of the ST-LS1 promoter. The levels of aphid mortality after 5 days of the inoculation in the assessed transgenic lines ranged from 20 to 53.3%. The range of the aphid population in transgenic plants 15 days after inoculation was between 17.0 +/- 1.43 (ST2) and 36.6 + 0.99 (35S3) aphids per plant, which corresponds to 24.9-53.5% of the aphid population in non-transformed plants. The results of our study suggest that GNA expressed in transgenic potato plants confers a potential tolerance to aphid attack, which appears to be an alternative against the use of pesticides in the future.
外文关键词：Aphid; GNA; Transgenic potato; Insect resistance; Leaf and stem-specific promoter
作者：Mi, Xiaoxiao; Liu, Xue; Yan, Haolu; 等
作者单位：Gansu Agr Univ
期刊名称：COMPTES RENDUS BIOLOGIES
期刊影响因子：1.064
出版年份：2017
出版刊次：1
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