中文摘要：开发食品中黄曲霉毒素B1（AFB1）的检测方法对保证食品质量和安全非常重要。大多数AFB1的检测仍然靠传统方法（如抗体ELISA试验、高效液相色谱-HPLC）；如今已开发了用作田间分析筛选工具的生物传感器法。与免疫学/ELISA试验或色谱法相比,生物传感器为现场测试提供了快速、灵敏、稳健而经济的定量方法。本研究提出了一种基于Vero细胞的生物传感器，膜经抗AFB1的抗体工程化，用作生物识别元件，与AFB1分子在金纳米粒/丝网印刷电极（SPEs）(三电极系统)上进行反应。研究发现本方法具有良好的灵敏度（R2 = 0.87）和检测限(0.5 ng/mL)。
外文摘要：The development of methods for the detection of aflatoxin B1 (AFB1) in foods is a very important practice for ensuring food quality and safety. Most tests of AFB1 are still conducted with conventional methods (i.e. antibody-based ELISA tests, high performance liquid chromatography – HPLC); however biosensor methods are being developed to date as screening tools for field analysis. Compared to immunology/ELISA-like tests or chromatography methods, biosensors are able to provide rapid, sensitive, robust and cost effective quantitative methods for on-site testing. In this work we propose a cellular biosensor based on Vero cells, membrane engineered with anti-AFB1 antibody as the biological recognition element reacting with AFB1 molecules on gold nanoparticle/screen printed electrodes (SPEs) (three electrode system). In order to culture the cells on the SPEs surfaces the working electrodes were coated with poly-l-lysine to facilitate cell adhesion. The SPEs were connected to a potentiostat device through a transducer and chronoamperometric (CA) and cyclic voltammetric (CV) measurements were performed. Quantitative results obtained using the cellular biosensor method for AFB1 were compared to those obtained using the HPLC method in pistachio samples spiked with AFB1. The method displayed good sensitivity (r2 = 0.87) and detection limit (0.5 ng/mL).
外文关键词：Cellular biosensor；Membrane engineering；Aflatoxin B-1；Pistachio samples
作者：Mavrikou, S；Flampouri, E；Iconomou, D；Kintzios, S
作者单位：Agr Univ Athens
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：3
点击下载：开发基于膜工程化韦罗细胞与金纳米粒子丝网印刷电极表面的抗AFB1抗体的相互作用的细胞生物传感器检测黄曲霉毒素B1