一种集成了光电探测器阵列的POU微流化装置用于多重免疫分析:检测多个样品中的霉菌毒素组。

A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples

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中文摘要:要将POU分析装置实际应用成功,要求快速,操作简单,并在理想情况下能够多重检测多种分析物和样品。霉菌毒素的检测日益凸显,特别是当食品及其原料中的这些毒素如赭曲霉毒素A(OTA)、黄曲霉毒素B1(AFB1)及脱氧雪腐镰刀菌烯醇(DON)在欧盟受到严格的规定和建议时。本研究开发了一种带手动操作电磁阀的新型、简单、负压驱动装置,并利用该装置在约0.12mm2面积内通过探针的层流图案及随后通过HRP标记的抗体化学发光的产生同时免疫检测了这三种毒素。本研究开发的装置用于POU多重免疫分析简单有效并在本研究中用于霉菌毒素的筛选。
外文摘要:For a point-of-use analytical device to be successful in real-world applications, it needs to be rapid, simple to operate and, ideally, able to multiplex the detection of several analytes and samples. Mycotoxin detection in food and feedstock in particular has become increasingly relevant as these toxins, such as ochratoxin A (OTA), aflatoxin B1 (AFB1) and deoxynivalenol (DON), are subject to strict regulations and recommendations in the European Union. A novel, simple, negative pressure-driven device with manually operated magnetic valves was developed and the simultaneous immunodetection of these three mycotoxins was demonstrated via the laminar flow patterning of probes in an area of ≈0.12mm(2) and subsequent chemiluminescence generation via HRP-labeled antibodies. The three mycotoxins were detected in less than 20min at concentrations of 100ng/mL for OTA and DON and 3ng/mL for AFB1, spiked in a sample under analysis and simultaneously compared to a toxin-free reference and a standard contaminated with critical target concentrations. The on-chip optical detection was performed in a single acquisition step by integrating a microfabricated array of 25×25µm(2) hydrogenated amorphous silicon (a-Si:H) photosensors below the microfluidic chip. The device presented in this work is simple and effective for point-of-use multiplexing of immunoassays and was applied in this work to the screening of mycotoxins.
外文关键词:Magnets;Multiplexing;Patterning;Photoconductors;Mycotoxins;Laminar
作者:Soares, RRG;Santos, DR;Chu, V;Azevedo, AM;Aires-Barros, MR;Conde, JP
作者单位:Inst Engn Sistemas & Computadores Microsistemas
期刊名称:BIOSENSORS & BIOELECTRONICS
期刊影响因子:7.476
出版年份:2017
出版刊次:1
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  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2017-05-22