中文摘要：禾谷镰刀菌在加拿大引起小麦赤霉病（FHB）和玉米穗腐病（GER），并产生单端孢霉烯霉菌毒素污染粮食。加拿大中部安大略省玉米单端孢霉烯多样性及真菌种群结构鲜为人知。本文鉴定了2010-2012年从玉米（n=452）和小麦(n = 110)中分离的禾谷镰刀菌单端孢霉烯基因型。研究结果提供了加拿大玉米中禾谷镰刀菌株3-乙酰脱氧雪腐镰刀菌烯醇(3ADON)和15ADON的基准数据并用于评估玉米和小麦籽粒的霉菌毒素污染风险。了解真菌的遗传结构将有助于评估并培育抗小麦FHB和玉米GER的抗性品种/种质。
外文摘要：Fusarium graminearum causes fusarium head blight (FHB) of wheat and gibberella ear rot (GER) of corn in Canada and also contaminates grains with trichothecene mycotoxins. Very little is known about trichothecene diversity and population structure of the fungus from corn in Ontario, central Canada. Trichothecene genotypes of F. graminearum isolated from corn (n = 452) and wheat (n = 110) from 2010 to 2012 were identified. All the isolates were deoxynivalenol (DON) type. About 96% of corn isolates and 98% of wheat isolates were 15-acetyl deoxynivalenol (15ADON) type. The fungal population structures from corn (n = 313) and wheat (n = 73) were compared using 10 variable number tandem repeat (VNTR) markers. The fungal populations and subpopulations categorized based on host, cultivar groups, years and geography showed high gene (H = 0.818-0.928) and genotypic (GD = 0.999-1.00) diversity. Gene flow was also high between corn and wheat population pairs (Nm = 8.212), and subpopulation pairs within corn (Nm = 7.13-23.614) or wheat (Nm = 19.483) populations. Phylogenetic analysis revealed that isolates from both hosts were F. graminearum clade 7. These findings provide baseline data on 3-acetyl deoxynivalenol (3ADON) and 15ADON profiles of F. graminearum isolates from corn in Canada and are useful in evaluating mycotoxin contamination risks in corn and wheat grains. Understanding the fungal genetic structure will assist evaluation and development of resistant cultivars/germplasm for FHB on wheat and GER on corn.
外文关键词：fusarium head blight；gene flow；gibberella ear rot；mycotoxins
作者：Burlakoti, RR；Tamburic-Ilincic, L；Limay-Rios, V；Burlakoti, P
作者单位：Weather INnovat Consulting LP
期刊名称：PLANT PATHOLOGY
期刊影响因子：2.383
出版年份：2017
出版刊次：1
点击下载：比较加拿大中部安大略省玉米和小麦禾谷镰刀菌的种群结构及单端孢霉烯霉菌毒素