中文摘要：本文对表达抗虫Cry1Ac和SCK基因的转基因水稻的三代生殖毒性进行了评估。作者将120只SD大鼠随机分为三组，分别对三组大鼠饲喂转基因水稻饮食（GM组）、双亲控制水稻饮食（PR组）和AIN-93控制饮食（作为阴性对照）。作者分别对每一代大鼠的体重、食物消耗量、生殖数据、血液学指标、血清化学指标、器官相对重量和病理组织学进行了检查。研究发现，虽然三组大鼠的血液学和血清化学的参数、生殖数据有所不同，但三组大鼠的血液学和血清生化学指标、器官重量/体重指标均在正常范围内或无明显的不利变化。另外，三组大鼠中没有发现或被视为与治疗相关的宏观或组织学的不良反应。研究结果表明，三代表达Cry1Ac和SCK基因的转基因水稻对大鼠的生殖系统没有意外的副作用。
外文摘要：In the present work, we evaluated the three generation reproductive toxicity of the genetically modified rice with insectresistant cry1Ac and sck genes. 120 Sprague-Dawley (SD) rats were divided into three groups which were fed with genetically modified rice diet (GM group), parental control rice diet (PR group) and AIN-93 control diet (both used as negative control) respectively. Bodyweight, food consumption, reproductive data, hematological parameters, serum chemistry, relative organ weights and histopathology for each generation were examined respectively. All the hematology and serum chemistry parameters, organ/body weight indicators were within the normal range or no change to the adverse direction was observed, although several differences in hematology and serum chemistry parameters (WBC, BUN, LDH of male rat, PLT, PCT, MPV of female rats), reproductive data (rate of morphologically abnormal sperm) were observed between GM rice group and two control groups. No macroscopic or histological adverse effects were found or considered as treatment-related, either. Overall, the three generation study of genetically modified rice with cry1Ac and sck genes at a high level showed no unintended adverse effects on rats's reproductive system.
外文关键词：Genetically modified rice; cry1Ac; sck genes; Three generation study; Feeding study; Toxicology
作者：Hu, Yichun; Zhuo, Qin; Gong, Zhaolong; 等
作者单位：Chinese Ctr Dis Control & Prevent China CDC
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.584
出版年份：2017
出版刊次：1
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