中文摘要：pUC-RICE5是一种新型的标准参考质粒，本文将它作为转基因水稻（Bt63、Kemingdaol、Kefeng6、Kefeng8和LLRice62）事件特异性定性和定量检测的阳性对照和校准器。pUC-RICE5含有水稻特异性内源参考基因（蔗糖磷酸合酶）和五种转基因水稻的片段。作者通过用pUC-RICE5修改定性PCR分析方法，来研发一个检测限与1-10份水稻单倍体基因组相互关联的定量方法。在定量PCR分析中，二乘回归系数的范围为0.993-1.000；标准差和相对标准偏差的范围分别为0.02 -0.22 和0.10%-0.67%。韩国食品药品安全部已验证了该方法及研究结果，并建议将它作为检测物种转基因水稻的常用方法。
外文摘要：One novel standard reference plasmid, namely pUC-RICE5, was constructed as a positive control and calibrator for event-specific qualitative and quantitative detection of genetically modified (GM) rice (Bt63, Kemingdaol, Kefeng6, Kefeng8, and LLRice62). pUC-RICE5 contained fragments of a rice-specific endogenous reference gene (sucrose phosphate synthase) as well as the five GM rice events. An existing qualitative PCR assay approach was modified using pUC-RICE5 to create a quantitative method with limits of detection correlating to approximately 1-10 copies of rice haploid genomes. In this quantitative PCR assay, the square regression coefficients ranged from 0.993 to 1.000. The standard deviation and relative standard deviation values for repeatability ranged from 0.02 to 0.22 and 0.10% to 0.67%, respectively. The Ministry of Food and Drug Safety (Korea) validated the method and the results suggest it could be used routinely to identify five GM rice events.
外文关键词：Genetically modified rice; Standard reference plasmid; Qualification; Quantification; Polymerase chain reaction
作者：Kim, Jae-Hwan; Park, Saet-Byul; Roh, Hyo-Jeong; 等
作者单位：Kyung Hee Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：4
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