中文摘要：转GmTMT基因玉米是超表达大豆γ-生育酚甲基转移酶（γ-TMT）基因的转基因玉米品种。γ-TMT基因导入Zhen58玉米中可以编码GmTMT2a蛋白，该蛋白可将γ-生育酚转化为α-生育酚。在超表达GmTMT2a基因的转基因玉米中，α-生育酚的含量显著增加。本文的研究目的是通过为期90天的亚慢性动物喂养研究，来调查转GmTMT基因玉米的潜在影响。在90天内，将转GmTMT基因玉米和Zhen58玉米分别以低（12.5%）、中（25%）、高（50%）三种浓度放入日粮中饲喂SD大鼠。阴性对照组的大鼠饲喂普通玉米日粮。研究发现，与饲喂Zhen58玉米相比，饲喂转GmTMT基因玉米对大鼠的体重、饲料消耗量、临床化学、血液学、绝对和相对器官重量等没有与治疗相关的副作用；且在尸检和组织病理学检查上没有发现与治疗相关的变化。研究结果表明，转GmTMT基因玉米与常规非转基因玉米具有一样的安全性和营养价值。
外文摘要：GmTMT transgenic maize is a genetically modified maize plant that overexpresses the gamma-tocopherol methyltransferase (gamma-TMT) from Glycine max (Gm). The gamma-TMT gene was introduced into maize line Zhen58 to encode the GmTMT2a protein which can convert gamma-tocopherol into alpha-tocopherol. Over expression of GmTMT2a significantly increased the alpha-tocopherol content in transgenic maize. The present study was designed to investigate any potential effects of GmTMT maize grain in a 90-day subchronic rodent feeding study. Maize grains from GmTMT or Zhen58 were incorporated into rodent diets at low (12.5%), medium (25%) or high (50%) concentrations and administered to Sprague-Dawley rats (n = 10/sex/group) for 90 days. The negative control group of rats (n = 10/sex/group) were fed with common maize diets. Results from body weights, feed consumption, clinical chemistry, hematology, absolute and relative organ weights indicated no treatment-related side effects of GmTMT maize grain on rats in comparison with rats consuming diets containing Zhen58 maize grain. In addition, no treatment related changes were found in necropsy and histopathology examinations. Altogether, our data indicates that GmTMT transgenic maize is as safe and nutritious as its conventional non-transgenic maize.
外文关键词：Transgenic maize; Toxicity; Feeding study; Rats
作者：Fang, Jin; Feng, Yongquan; Zhi, Yuan; 等
作者单位：Natl Ctr Food Safety Risk Assessment
期刊名称：REGULATORY TOXICOLOGY AND PHARMACOLOGY
期刊影响因子：2.227
出版年份：2017
出版刊次：4
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