中文摘要：本文对采用改进的快速极性农药提取方法对生菜和芹菜中极性农药进行分析的实时直接分析法进行了评价。为了获得可同时检测多种混合样本中化合物所需特异性，本文将实时直接分析法与高分辨质谱法联用进行检测。本文对解吸温度、扫描速度、实时直接分析离子源与MS入口之间的距离等进行了优化，然后对两个不同的质量分析器和进样配件进行了评价。结果发现，采用伯胺（PSA）的额外清洗步骤可提高快速极性农药提取方法的灵敏度，而源碎片是主要限制因素，总之，对至少七种化合物（杀草强、灭蝇胺、丙酰胺、三聚氰胺、二乙醇胺、三乙醇胺和三唑）来讲，快速极性农药提取方法是一个快速、具有定量功能的可靠工具。检测限范围为20-60μg/kg，强化样本回收率范围为71-115%，相对标准差小于18%。
外文摘要：Direct analysis in real time (DART) was evaluated for the determination
of a number of highly polar pesticides using the Quick Polar Pesticides Extraction (QuPPe) method. DART was hyphenated to high resolution mass spectrometry (HRMS) in order to get the required selectivity that allows the determination of these compounds in complex samples such as lettuce and celery. Experimental parameters such as desorption temperature, scanning speed, and distances between the DART ion source and MS inlet were optimized. Two different mass analyzers (Orbitrap and QTOF) and two accessories for sample introduction (Dip-it((R)) tips and QuickStrip (TM) sample cards) were evaluated. An extra clean-up step using primary-secondary amine (PSA) was included in the QuPPe method to improve sensitivity. The main limitation found was in-source fragmentation, nevertheless QuPPe-DART-HRMS proved to be a fast and reliable tool with quantitative capabilities for at least seven compounds: amitrole, cyromazine, propamocarb, melamine, diethanolamine,triethanolamine and 1,2,4-triazole. The limits of detection ranged from 20 to 60 mu g/kg. Recoveries for fortified samples ranged from 71 to 115%, with relative standard deviations < 18%. (C) 2017 Elsevier B.V. All rights reserved. 
 
外文关键词：Polar pesticides, Direct analysis in real time, High resolution mass spectrometry, Vegetable samples, Quick Polar Pesticides Extraction
作者：Lara, Francisco J.; Chan, Danny; Dickinson, Michael; Lloyd, Antony S.; Adams, Stuart J.
作者单位：Univ Granada, Dept Analyt Chem, Fac Sci, Campus Fuentenueva S-N, E-18071 Granada, Spain.
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：5
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